摘要
以1,3-二(4-氨基-吡啶)丙烷四氟硼酸盐离子液体修饰石墨烯纳米复合物,负载酪氨酸酶至玻碳电极表面构建双酚A电化学传感器.优化了滴涂量、检测电位、pH值等实验条件对传感器响应性能的影响.结果表明,传感器的安培响应电流与双酚A浓度在1.0×10^-9~3.8×10^-5 mol/L范围内具有良好的线性关系,检出限为3.5×10^-10 mol/L(RSN=3).用于矿泉水瓶中溶出双酚A含量测定,结果与高效液相色谱一致.
Graphene-1,3-di(4-amino-1-pyridinium)propane tetrafluoroborate ionic liquid nanocomposite(Gr-DAPPT)modified on a glassy carbon electrode and then tyrosinase(Tyr)was immobilized onto the electrode to fabricate a biosensor.Experimental conditions including the volume of Tyr-Gr-DAPPT solution,the applied potential,and pH value were optimized for bisphenol A(BPA)determination.The biosensor exhibited a linear response to BPA in the concentration range from 1.0×10^-9 to 3.8×10^-5 mol/L.The detection limit was calculated to be 3.5×10-10 mol/L(RSN=3).Practical application of the biosensor was demonstrated by determining BPA leaching from commercial plastic drinking bottles.The result was consistent with that obtained by high performance liquid chromatography.
出处
《信阳师范学院学报(自然科学版)》
CAS
北大核心
2015年第4期546-549,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
国家自然科学基金项目(21275166)
国家质量监督检验检疫总局科研项目(2013Qk286)