基于酪氨酸酶/离子液体-石墨烯纳米复合界面的双酚A电化学传感器

Electrochemical Sensor for Bisphenol A Based on Tyrosinase/ionic Liquid-graphene Nanointerface

以1,3-二（4-氨基-吡啶）丙烷四氟硼酸盐离子液体修饰石墨烯纳米复合物,负载酪氨酸酶至玻碳电极表面构建双酚A电化学传感器.优化了滴涂量、检测电位、pH值等实验条件对传感器响应性能的影响.结果表明,传感器的安培响应电流与双酚A浓度在1.0×10^-9~3.8×10^-5 mol/L范围内具有良好的线性关系,检出限为3.5×10^-10 mol/L（RSN=3）.用于矿泉水瓶中溶出双酚A含量测定,结果与高效液相色谱一致.

Graphene-1,3-di（4-amino-1-pyridinium）propane tetrafluoroborate ionic liquid nanocomposite（Gr-DAPPT）modified on a glassy carbon electrode and then tyrosinase（Tyr）was immobilized onto the electrode to fabricate a biosensor.Experimental conditions including the volume of Tyr-Gr-DAPPT solution,the applied potential,and pH value were optimized for bisphenol A（BPA）determination.The biosensor exhibited a linear response to BPA in the concentration range from 1.0×10^-9 to 3.8×10^-5 mol/L.The detection limit was calculated to be 3.5×10-10 mol/L（RSN=3）.Practical application of the biosensor was demonstrated by determining BPA leaching from commercial plastic drinking bottles.The result was consistent with that obtained by high performance liquid chromatography.