摘要
利用水热法制备了还原氧化石墨烯-硅胶复合物,将其用于固相萃取柱制备,并与高效液相色谱相结合,建立了牛奶中磺胺类药物测定的新方法.对萃取条件进行了优化,优化条件为:0.1 mL的甲醇-乙酸(V∶V′=1∶1.5)混合液为洗脱剂,75mL的样品溶液.在优化条件下,目标物的线性范围为0.1~100μg/L,检出限为0.027~0.078μg/L(RSN=3).新方法用于牛奶样品分析,加标回收率为88.3%~113.6%.
Reduced graphene oxide-silica gel composites were prepared by hydrothermal method and used as adsorbents of solid phase extraction column.A new method was developed for sensitive determination of sulfonamides in milk by solid-phase extraction coupled with high-performance liquid phase chromatography.The extraction conditions were optimized as follows:0.1mL methanol-acetic acid solution(V∶V′=1∶1.5)was selected as eluent and the volume of sample were 75 mL.The developed method showed a wide linear range of 0.1~100μg/L and low detection limits of 0.027~0.078μg/L.The method was applied to determine sulfonamides in milk with recoveries of 88.3%~113.6%.
出处
《信阳师范学院学报(自然科学版)》
CAS
北大核心
2015年第4期559-562,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
河南省科技计划项目(132300410060)
关键词
还原氧化石墨烯-硅胶复合物
高效液相色谱
固相萃取
灵敏检测
reduced graphene oxide-silica gel composites
high-performance liquid phase chromatography
solid-phase extraction
sensitive determination