生长抑制因子3在肝癌组织的表达及其临床意义

Clinicopathological significance of the expression of inhibitor of growth 3 in primary hepatocellular carcinoma

目的探讨生长抑制因子3（ING3）在原发性肝癌中的表达和意义。方法免疫组织化学检测105例原发性肝癌组织中ING3的表达Kendall’stan法进行相关性分析；Kaplan-Meier法绘制生存曲线，采用Log-rank检验分析；Cox比例风险回归模型分析独立预后影响因素。结果与癌旁组织比较，ING3在肝癌组织中低表达。肝癌组织中ING3低表达与肝癌肿瘤数量、分化程度、巴塞罗那临床肝癌分期标准（BCLC）、TNM分期、有无静脉浸润和有无“卫星”结节等临床病理因素之间有显著相关性（P〈0．05），而与患者性别、年龄等其他临床病理因素之间无明显相关（P〉0．05）。Cox回归分析显示ING3低表达是肝癌患者总生存率的独立影响因素[风险比（HR）：2．742；95％可信区间（CI）：1．270～9．959，P〈0．01]，也是患者无复发生存的独立影响因素（HR：1．724；95％CI：0．856～3．940，P〈0．01）。Kaplan-Meier生存分析表明，与ING3高表达的患者比较，ING3低表达的患者总体生存（P〈0．05）和无复发生存均较差（P〈0．05）。结论ING3的表达降低参与原发性肝癌的发生发展，是原发性肝癌的独立预后影响因素。

Objective To investigated the expression of inhibitor of growth 3 （ ING3 ） and its clin- ieopathological significance in primary hepatoeellular carcinoma. Methods Immunohistochemistry was employed to detect the expression of ING3 in primary hepatoeellular carcinoma tissues of 105 patients. Cor- relation between variables was analyzed by Kendall＇ s tan method. Survival curves were plotted using Kap- lan - Meier method with Log - rank test. Cox proportional hazard model was used to evaluate the independ- ent prognostic factors. Results Compared with paracancerous tissues the expression level of ING3 de- creased in tumor tissues. Low expression of ING3 significantly correlated with the clinical parameters inclu- ding tumor number, differentiation, Barcelona Clinical Liver Classification （BCLC） , TNM stage, venous invasion, satellite nodules （ P 〈 0. 05 ）, and showed no significant correlation with other clinical parameters including age, sex and so on. Cox regression analysis indicated that ING3 expression was independently in- fluencing factor of overall survival [ hazard ratio （HR） ： 2. 742 ; 95% confidence interval （CI） ： 1. 270 - 9. 959 ; P 〈 0.01 ] and recurrence - free survival （ HR ： 1. 724 ; 95 % CI： 0. 856 - 3. 940 ; P 〈 0. 01 ） in liver cancer patients. Kaplan - Meier survival analysis showed that patients with low ING3 expression had poorer overall survival （ P 〈 0. 05） and poorer recurrence - free survival （ P 〈 0. 05 ） than those with high ING3 expression. Conclusion Low expression of ING3 took part in the development of primary hepatocel- lular carcinoma, and was independent prognostic factor in primary liver cancer patients.