CD4＋CD25＋Foxp3＋调节性T细胞在乳腺癌组织中的变化及其意义

Expression of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cells in breast cancer tissue and clinical signifi-cance

目的观察CIM^＋CD25^＋Foxp3^＋调节性T细胞在乳腺癌组织中的变化，并探讨其意义。方法收集乳腺癌组织67例，乳腺纤维腺瘤组织32例，正常乳腺组织26例，采用免疫组织化学法检测Foxp3^＋调节性T细胞及白细胞介素（IL）-10阳性肿瘤细胞在上述组织中的数量；采用反转录一聚合酶链反应（RT-PCR）的方法检测1％xp3在不同组织中的基因表达，分析其与肿瘤临床病理特点之间的关系。结果Foxp3^＋调节性T细胞及IL-10阳性肿瘤细胞在乳腺癌组织中的数量显著多于乳腺纤维腺瘤组织及正常乳腺组织，差异有统计学意义（P〈0．01）；肿瘤组织中Foxp3的相对表达量较乳腺纤维腺瘤组织及正常乳腺组织明显增强，差异有统计学意义（P〈0．01）；肿瘤组织中Foxp3的表达量与组织学分级、淋巴结转移、TNM分期明显相关（P〈0．05），与年龄无明显相关（P〉0．05）；IL-10表达量与肿瘤分化水平、临床分期明显相关（P〈0．05）。结论CIM^＋CD25^＋Foxp3^＋调节性T细胞可能是通过Foxp3的表达及IL-10等抑制性细胞因子的表达发挥肿瘤免疫抑制作用。

Objective To study the changes of CD4^＋ CD25^＋ Foxp3 ^＋ regulatory T ceils in breast cancer and the clinical significance. Methods Sixty - seven cases of breast cancer tissues, 32 cases of breast fibroadenoma tissues, and 26 cases of normal breast gland tissues were collected. The immunohisto- chemistry was used to detect the number of Foxp3 ＋ regulatory T cells and interleukin （IL） - 10 positive cells. Reverse transcriptase -polymerase chain reaction （RT- PCR） was used to deetect the expression of Foxp3 in different tissues. The correlation between Foxp3 and IL - 10 with clinicopathological features was analyzed. Results The number of Foxp3^＋ regulatory T cells and IL - 10 positive tumor cells in breast cancer tissues was significantly greater than in the breast fibroadenoma and normal breast tissues （ P 〈 0. 01 ）. The Foxp3 expression in breast cancer tissue was significantly increased as compared with that in breast fibroadenoma and normal breast tissues （ P 〈 0. 01 ）. The expression of Foxp3 in breast cancer pa- tients was correlated with the histological grade, lymph node metastasis and TNM stage （ P 〈 0. 05 ）, but not with age （P 〉0. 05）. The expression of IL - 10 was correlated with the histological grade, and TNM stage （P 〈 0. 05）. Conclusion CIM^＋ CD25^＋ Foxp3^＋ regulatory T cells inhibit tumor immunity through the expression of Foxp3 and some cytokines.