骨髓telocytes的形态及免疫表型特征（英文）

Phenotypical and morphological features of bone marrow telocytes

目的探讨骨髓telocytes(TCs)的免疫表型特征。方法以C57BL/6J小鼠为研究对象,通过扫描电镜原位观察骨髓TCs的形态学特征;分离培养骨髓TCs并利用相差显微镜和激光扫描共聚焦显微镜观察。结果扫描电镜下,骨髓TCs胞体向外发出极其细长的突起(telopodes,Tps),相邻Tps通过直接接触形成相互联系。细胞培养发现TCs胞体较小,呈椭圆形,胞体发出细长的突起(250.33μm),由膨大的粗段(Podom)与细段(Podomer)交替组成。亚甲蓝染色、吉姆萨染色和詹纳斯绿染色均提示为典型的TCs。免疫荧光染色发现CD34、CD117、CD45、CD73和CD90在骨髓TCs中为阳性表达。结论首次成功分离和培养骨髓TCs,并对其形态学和免疫表型特征进行了描述。

Objective The present study aimed to show the molecular features of telocytes （ TCs ）. Methods TCs were isolated from bone marrow of C57BL/6J mice. TCs in vivo were observed under scanning election microscope （SEM） and TCs in culture were evaluated via phase-contrast microscope, vital cell staining and laser scanning confocal microscope （LSCM）. Results Bone marrow TCs in situ extended specific long and thin telopodes （Tps） and interacted through direct contact along longitudinal axis. In culture, TCs were identified with small oval cell bodies and very long Tp（250.33 t^m） with the alternation of podoms and podomers. Base on cultured TCs, the population indicated distinctive structures by a combination of vital dyes.methylene blue,Giemsa and Janus green B. Furthermore, the cellular phonotype in bone marrow TCs was investigated, with positive expression of CD34, CDl17, CD45,CD73 and CD90. Conclusions Bone marrow TCs were isolated and cultured successfully,and the primary biochemical features of the population were clarified for the first time.