肿瘤坏死因子α与白介素17在银屑病中的表达

The common expression of TNF-α and IL-17 in psoriasis and its significance

目的:检测TNF-α与IL-17在银屑病皮损中的表达。方法:ELISA检测68例银屑病患者与28例对照(非银屑病患者)血清TNF-α和IL-17的表达;免疫印迹法检测局部皮损、患者正常皮肤及对照者皮肤中TNF-α和IL-17的表达;qT-PCR检测TNF-α和IL-17的mRNA表达。结果:银屑病患者血清TNF-α、IL-17的表达均高于对照组(P<0.05);皮损组织中TNF-α、IL-17蛋白及mRNA的表达均高于患者正常皮肤及对照组皮肤(均P<0.05)。结论:TNF-α与IL-17在银屑病的发展中可能相互作用。

Objective：To detect the expression of TNF-αand IL-17 in the patients with psoriasis. Meth-ods：The serum levels of TNF-α and IL-17 were detected in 68 patients with psoriasis and 28 controls from cosmetic surgery department. The expression of protein TNF-αand IL-17 in the patients’ lesions and normal skin of the patients as well as the skin of normal controls was detected by ELISA and the mRNA of TNF-αand IL-17 was detected by qT-PCR. Results：The levels of serum TNF-αand IL-17 were higher in the patients than in controls. The levels of protein and mRNA expression of TNF-αand IL-17 were higher in the patients’ lesions than in the normal skin of the patients and controls. Conclusion：TNF-αand IL-17 may be interacted in the progress of psoriasis.