毛细管电泳在手性氨基酸拆分中的研究进展

Reasearch Advances in Chiral Separation of Amino Acids by Capillary Electrophoresis

毛细管电泳(capillary electrophoresis,CE)作为一种强有力的手性分离技术,由于操作简单、试剂消耗少及柱效高等优点,受到广泛关注,是近年来手性分离领域的研究热点.氨基酸是组成蛋白质的基本单元,且大多数氨基酸具有手性中心,手性氨基酸是生命体系的一个重要特征.具有手性中心的氨基酸,其对映体间的生物活性往往存在着较大的差异,因此,氨基酸的手性拆分对了解人体及动物生命活动起着举足轻重的作用.主要总结了近5年来毛细管电泳的3种分离模式(毛细管区带电泳、胶束电动毛细管色谱、毛细管电色谱)在氨基酸手性拆分中的发展和应用.

Capillary electrophoresis （CE）attracted widespread attention, because of its simple operation, minimal consumption of chemicals and high separation efficiency. It is also the research focus in recent years due to its powerful chiral separation ability. Amino acids are the basic modules of proteins and most of which have chiral centers. Chiral amino acid is an important feature of biological systems. Comparatively large difference in biological activity exists among the amino acid enantiomers. Therefore, chiral separation of amino acids plays an important role in understanding the life activities of both human and animal. In article the development and applications of chiral separation of amino acids by three separation modes of CE, including capillary zone electrophoresis （ CZE）, micellar electrokinetic capillary chromatography （MEKC） and capillary electrochromatography （CEC） in the last five years are reviewed.