高温胁迫对不同品种羽衣甘蓝幼苗叶的生理特性及SOD、POD同工酶的影响

Effect of High Temperature Stress on Physiological Characteristics and POD,SOD Isoenzyme in Brassica oleracea Seedlings

以4个不同品种的羽衣甘蓝(Brassica oleracea var.acephala)幼苗为试材,研究了在高温胁迫8d(39±0.5)℃/(28±0.5)℃(昼/夜)后,羽衣甘蓝幼苗叶片一些抗逆境的生理生化指标及SOD、POD同工酶变化,以探讨不同羽衣甘蓝植物对高温的适应性。结果表明:随胁迫时间的延长,不同品种的羽衣甘蓝的叶绿素含量显著下降,而幼苗叶MDA、脯氨酸含量与质膜透性含量增加;在处理后2d,SOD、POD含量有较大幅度的升高,但第4天后开始出现下降,不同品种之间有显著差异;试验表明"名古屋"、"京冠红2号"比"红鸥"和"叶牡丹"有较强的耐热性;SOD、POD同工酶研究表明,"名古屋"和"叶牡丹"的SOD同工酶谱带具有相似性,而与"红鸥"与"京冠红2号"不同;"名古屋"和"京冠红2号"的POD同工酶谱带相似,而"叶牡丹"与"红鸥"的POD谱带相似,但"名古屋"和"京冠红2号"与"叶牡丹"和"红鸥"的POD谱带差异较大。

Four different regions of the kales（Brassica oleracea）seedlings were used as materials,treated at（39±0.5）℃/（28±0.5）℃（day/night temperature）for 8days,and several related physiological indexes and SOD,POD isoenzyme in kale seedlings were determined,in order to discuss their plant adaptability under high temperature conditions.The results showed that the relative electric conductivity content,contents of malondialdehyde（MDA）,and free proline were gradually increased,but chlorophyll content decreased with the time of high temperature continued in different varieties.During the early 2day after treatment,superoxide dismutase（SOD）and peroxidase（POD）activities increased dramatically,while fourth days later,they began to decline.But there were significant differences among different varieties.Research data showed that‘Nagoya＇and ‘Jingguan red 2＇had stronger heat resistance than the ‘Red gull＇and ‘Leaf peony＇.According to the analysis of POD,SOD isoenzyme bands,the band of SOD isoenzyme in‘Nagoya＇and‘Leaf peony＇were similar,and ‘Red gull＇and ‘Jingguan red 2＇were different.The bands of POD isoenzyme were similar between‘Nagoya＇and ‘Jingguan red 2＇,while ‘Leaf peony＇and ‘Red gull＇POD bands were similar,the bands of POD isoenzyme were difference between‘Nagoya＇,‘Jingguan red 2＇and‘Leaf peony＇,‘Red gull＇.