Optimization on Fermentation Conditions of Orange Vinegar by Box-behnken Design

应用Box-behnken响应面优化柑桔果醋发酵工艺研究（英文）

The optimal process for orange vinegar was investigated in order to explore the effect of process parameters and obtain the superior process parameters.The process for orange vinegar was optimized using single-factor methodology and Box-behnken methodology, and the effect of process parameters was analyzed in the light of response surface plots. Results were as follows. The optimal process of alcohol fermentation was as follows： original sugar degree of 16%, yeast inoculum size of 5% and p H value of 3.5 through single-factor experiment. The optimal fermentation process of acetic acid optimized by response surface methodology was as follows： inoculum size of 8.52%, original alcohol content of 6.0% and p H value of 3.3, in the optimal condition the yield of acetic acid was 53.11 g/L. Single factor action and the interaction of two factors were presented on the yield of acetic acid.The effects of inoculum size and p H were extremely significant, and that of original alcohol content significant. The interaction of inoculum size and original alcohol content was non-significant, that of inoculum size and p H also non-significant. On contrast, original alcohol content and p H had significant interaction.

[目的]采用Box-Behnken设计进行工艺优化,研究柑桔果醋的酒精发酵和醋酸发酵工艺,为柑桔果醋的工业化应用奠定基础。[方法 ]以柑桔为原料,初始糖度、初始pH值、接种量3个因素为主要影响因素,以醋酸产量为为评价指标进行Box-Behnken设计试验,考察最佳工艺。[结果]柑桔果醋的酒精发酵优化工艺参数为:初始糖度为16%、接种量为5%、初始p H为3.5。醋酸发酵优化工艺参数为:接种量为8.52%、初始酒精度为6.02%、发酵pH为3.3,该工艺下醋酸产量53.11 g/L。响应面优化试验中接种量对醋酸的影响极显著,初始酒精度对醋酸的影响显著,发酵pH对醋酸的影响极显著;接种量与初始酒精度的交互作用对醋酸的影响不显著,接种量与发酵pH交互影响醋酸不显著,初始酒精度与发酵p H的交互作用对醋酸的影响显著。[结论]采用BoxBehnken设计优化柑桔果醋的发酵工艺稳定可行,为进一步地柑桔果醋的工业化应用奠定基础。