摘要
为了通过DNA软件分析河鲀鱼保守序列,试验设计了河鲀鱼扩增引物,首次建立了利用高分辨熔解曲线方法快速鉴定红鳍东方鲀与星点东方鲀。结果表明:该方法不仅可以快速鉴定河鲀鱼,而且可以通过高分辨熔解曲线区分红鳍东方鲀与星点东方鲀,正确率为100%。
To analyze the conserved sequence of puffer fish using the DNA software, the amplification primers of puffer fish were designed in the test. And then a high - resolution melting curve method was firstly developed to rapidly identify Takifugu rubripes and Takifugu niphobles. The results showed that the method not only could identify the puffer fish, but also could distinguish both Takifugu rubripes and Takifugu niphobles by high -resolution melting curve, and its accuracy reached 100%.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2015年第10期230-232,300,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家质量监督检验检疫总局项目(2011IK009)
关键词
高分辨熔解曲线
河鲍鱼
鉴定
红鳍东方鲀
星点东方鲀
high - resolution melting curve
puffer fish
identification
Takifugu rubripes
Takifugu niphobles
