玉米花粉中营养物质的傅里叶变换红外光谱特征

Discrimination and Characterization of Nutrient Substance in Corn Pollen Using Fourier Transform Infrared Spectroscopy

为了研究玉米花粉中的蛋白质和多糖等营养物质，采用傅里叶变换红外光谱结合曲线拟合技术分析曲辰9号和靖丰3号两种杂交玉米花粉．玉米花粉的傅里叶变换红外光谱显示蛋白质的特征吸收峰酰胺Ⅰ带、酰胺Ⅱ带和酰胺Ⅲ带出现在1653cm-1、1546cm-1和1241cm-1附近，多糖特征吸收峰出现在1078cm-1、1056cm-1、1030cm-1和995cm-1附近．对比它们的傅里叶变换红外光谱的蛋白质和多糖特征吸收带，两种玉米花粉存在一定差异，表明它们所含的蛋白质和多糖含量不同．对1900～950cm-1吸收带实施傅里叶自去卷积和二阶导数处理后再进行曲线拟合分析，剥离出因光谱叠加而掩盖的特征吸收子峰．子峰信息显示曲辰9号玉米花粉中多糖的含量高于靖丰3号玉米花粉，而蛋白质的含量关系则相反．研究表明，傅里叶变换红外光谱技术可为玉米花粉中营养物质的研究提供一种便捷的手段．

To investigate the protein, polysaccharide and other nutrient substance in corn pollen, Fourier transform infrared spectroscopy combined with curve-fitting analysis was used to investigate two species of hybrid corn pollen, which called Quchen-9 and Jingfeng-3. The Fourier transform infrared spectra of corn pollen presented the characteristic absorption bands of protein and polysaccharide obviously. The peaks at 1 653 cm 1, 1 546 cm-1 and 1 241 cm 1 were assigned to amide-I band, amide-II band and amide-III band respectively, which come from protein of corn pollen. The peaks at I 078 cm-1, 1 056 cm-1, 1 030 cm-~ and 995 cm-1 were characteristic absorption bands of pollen polysaccharide. Compared with these characteristic absorption bands at the spectra of Quchen 9 and Jingfeng 3, there were obvious differences between the two species of corn pollen. It indicated that the concentration of protein and polysaccharide in corn pollen had difference between the species of Quchen-9 and Jingfeng-3. Fourier self-deconvolution and second derivative of the 1 900 - 950 cm-1 band was followed by curve- fitting analysis to generate the underlying multi-component peaks. The relative area percentages of multi- component peaks showed that the polysaccharide content in the corn pollen of Quchen-9 was higher than that of the Jingfeng-3. However, the relationship of protein content was reverse. The present results suggest that Fourier transform infrared spectroscopy is a promising method to identify nutrient substancein corn pollen.