百两茶提取物对东莨菪碱诱导小鼠学习记忆障碍的影响

Effect of Bailiang tea extract on scopolamine induced learning and memory impairment in mice

目的研究百两茶提取物对东莨菪碱诱导小鼠学习记忆障碍的影响。方法雄性BALB/C小鼠60只,随机分为6组,分别为空白对照组,东莨菪碱模型组,石杉碱甲片组,百两茶提取物低、中、高(100、200、400 mg·kg-1)剂量组,各组连续给药28 d,于试验前4 d除空白对照组外其余各组小鼠腹腔注射东莨菪碱2 mg·kg-1建立小鼠记忆障碍模型,随后进行跳台试验,记录跳下平台的潜伏期,进行水迷宫测试,记录动物5 min内登台时间。测试毕,处死小鼠,分离大脑皮质和海马,匀浆取上清液测大脑皮质和海马中超氧化物歧化酶(SOD)和乙酰胆碱酯酶(Ach E)的含量。结果百两茶提取物高剂量(400 mg·kg-1)能明显增加小鼠跳台潜伏期(P<0.05),缩短水迷宫小鼠登台时间(P<0.05),升高小鼠大脑皮质和海马组织SOD含量和抑制ACh E的活性。结论百两茶提取物具有明显改善学习记忆作用。

Objective To observe the effect of Bailiang tea extract on scopolamine induced learning and memory impairment in mice. Methods Sixty male BALB/C mice were randomly divided into 6 groups： a blank control group, a scopolamine model group, a huperzine group, and tea extract（100, 200 and 400 mg·kg- 1） groups. Each group was continuously administered 28 days. Except for the mice in blank group, other mice were injected scopolamine 2 mg·kg- 1 to establish memory impairment model 4 days. Animal platform experiment and water maze experiment were carried out respectively to record the jumping off platform of the incubation period and the stage time within 5 min. After testing, the cerebral cortex and hippocampus tissue were taken and homogenated. The supernatant was taken to detect the content of superoxide dismutase（SOD） and acetyl cholinesterase（Ach E）. ResultsThe Bailiang tea extract high dose（400 mg·kg- 1） significantly increased the incubation period platform in mice（P〈0.05）, and obviously shortened the water maze stage time in mice（P〈0.05）. It significantly increased SOD content in mice and inhibited the activity of ACh E in the cerebral cortex and hippocampus. Conclusion Bailiang tea extract can obviously improve the function of learning and memory.