山羊的绵羊肺炎支原体的分离和鉴定

Isolation and Identification of Mycoplasma ovipneumoniae from Goats

[目的]了解安徽省某山羊场肺炎症状的发生原因。[方法]从安徽省某山羊场采集病料样品,从山羊肺脏组织中分离致病菌并进行纯化,并通过形态学观察、生化试验、PCR对其进行鉴定。[结果]成功从患有肺炎的山羊肺脏组织中分离出致病菌,通过形态学观察、生化试验和PCR鉴定为绵羊肺炎支原体。[结论]该研究可为安徽省山羊支原体肺炎的防控及病原学研究提供科学依据。

[ Objective ] The aim was to understand the cause of pneumonia symptoms in a goat farm in Anhui Province. [ Method] The diseased samples were collected from a goat farm in Anhui Province. The pathogen was isolated and purified from lung tissue of goats. And the isolated pathogen was identified by using the morphological observation, biochemistry test and PCR. [ Result] The pathogen was successfully isolated from lung tissue of goats with pneumonia. And the isolated pathogen was identified as Mycoplasma ovipneumoniae by using morphological observation, biochemistry test and PCR. [ Conclusion ] The research could provide a scientific basis for the prevention, control and etiological study of mycoplasma pneetmonia of goats in Anhui Province.