枇杷花总黄酮、总三萜的大孔树脂制备工艺

Preparation of Flavonoids and Triterpenoids from Flowers of Eriobotrya japonica(Thunb.) Lindl by Macroporous Resins

以枇杷花中总黄酮、总三萜含量作为检测指标,考察D101、AB-8、聚酰胺3种大孔树脂对枇杷花提取物中总黄酮、总三萜的分离性能,考察4种不同干燥方法对枇杷花总黄酮、总三萜含量的影响。结果表明,大孔树脂AB-8作为最佳分离树脂,上样量2.1 m L、吸附时间4 h、洗脱速率2 BV/h、洗脱体积3 BV时,枇杷花洗脱液中总黄酮含量为74.82%,收率为76.56%,总三萜含量为15.57%,收率为46.48%;减压干燥作为最佳干燥方法,真空度0.07 MPa、温度45℃、干燥时间10 h时,纯化物中总黄酮含量为76.07%,收率为77.87%,总三萜含量为15.89%,收率为47.09%,含水量为4.07%。研究结果表明,此制备工艺可行,为枇杷花总黄酮、总三萜的开发利用提供理论依据。

The total flavonoids and triterpenoids of loquat flower extracts were purified with D101, AB-8 and polyamide resins and dried by atmospheric drying, vacuum drying, freeze drying and spray drying, respectively. The effectiveness of these methods was tested based on the content and yield of total flavonoids and triterpenoids. The results showed that AB-8 was the best macroporous resin for the separation and purification of flavonoids and triterpenoids, and vacuum drying was the best drying method. The optimal conditions for purification were determined as follows： sample volume, 2.1 mL; elution flow rate,2 BV/h;adsorption time,4h;and eluent volume, 3 BV. Under these conditions, the content and yield of flavonoids were 74.82% and 76.56% and the content and yield of triterpenoids were 15.57% and 46.48%, respectively. The optimal conditions for vacuum drying were obtained by 10 h drying at 45 ℃ with a vacuum degree of 0.07 MPa. Under the optimized drying conditions, flavonoids content was 76.07% with a yield of 77.87%, triterpenoids content was 15.89% with a yield of 47.09% and moisture content was 4.07%.