响应面试验优化粗糙脉胞菌中番茄红素的提取工艺

Optimization of Extraction of Intracellular Lycopene from Neurospora crassa by Response Surface Design

应用响应面分析法对粗糙脉孢菌(Neurospora crassa)中番茄红素的提取工艺进行优化。粗糙脉孢菌(Neurospora crassa)3.1607为发酵菌种发酵,研磨结合超声破碎法破碎菌体,以溶剂配比、浸提温度、浸提时间为影响因素,产物高效液相色谱分析得到提取量为响应值。通过响应面分析法得到最佳提取条件为浸提温度67.48℃、浸提时间2.51 h、溶剂配比(乙酸乙酯-丙酮,V/V)16∶25,此时番茄红素提取量最高可达71.227μg/g。按修正后的最佳工艺条件(67℃、2.5 h、溶剂配比2∶3)进行验证实验,实际提取结果为71.688μg/g,这与模型预测值吻合。

Response surface design was employed to optimize the extraction of intracellular lycopene from Neurospora crassa. The cultured cells of N. crassa 3.1607 were disrupted ultrasonically by milling for the extraction experiments which were designed to explore the effects of processing variables（extraction solvent, temperature and duration） on the extraction yield of lycopene. The optimal extraction conditions were determined as 67.48 ℃, 2.51 h and ethyl acetate-acetone（16：25, V/V） for temperature, time and extraction solvent, respectively. Under these conditions, the maximum predicted yield of lycopene of 71.227μg/g was obtained.Experiments conducted under the modified conditions（67℃, 2.5 h and ethyl acetate/acetone ratio = 2：3） resulted in an extraction yield of 71.688 μg/g, which was consistent with the model predicted value.