摘要
采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱(high performance liquid chromatography,HPLC)法及HPLC-质谱联用法测定葛仙米多糖的单糖组成,两种方法测定结果相互吻合。同时采用HPLC法测定了相关单糖的标准曲线,相关系数达到0.995以上,经计算,葛仙米多糖的单糖组成物质的量比甘露糖∶葡萄糖醛酸∶葡萄糖∶半乳糖∶阿拉伯糖为1∶1.12∶3.24∶1.72∶1.21。
Two analytical methods, namely high performance liquid chromatography（HPLC） and HPLC-mass spectrometry（HPLC-MS） using precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone were established for analyzing the monosaccharide composition of polysaccharide from Nostoc sphaeroides Kützing. Consistent results were obtained using both methods. The standard curve developed using monosaccharide standards by HPLC exhibited a correlation coefficient of greater than 0.995. According to the standard curves, the polysaccharide in N. sphaeroides was composed of manose,glucuronic acid, glucose, galactose and arabinose at a molar ratio of 1：1.12：3.24：1.72：1.21.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第18期89-92,共4页
Food Science
基金
国家自然科学基金面上项目(31171670)
关键词
葛仙米多糖
单糖
柱前衍生
高效液相色谱
质谱
Nostoc sphaeroides Kützing polysaccharide
monosaccharide
precolumn derivatization
HPLC
MS
