摘要
为探讨鲜食玉米中类胡萝卜素组成及含量差异,应用高效液相色谱-二极管阵列检测-大气压化学电离质谱技术对5个鲜食甜、糯玉米品种籽粒中主要类胡萝卜素进行鉴定与分析。结果表明:甜玉米‘晶甜3号’和‘晶甜5号’籽粒中分别鉴定出14、15种类胡萝卜素,糯玉米‘苏玉糯11号’、‘京甜紫花糯2号’和‘晶糯8号’中种类较少,其中新色素、花药黄质和13-顺式-5,6-环氧-叶黄素首次在鲜食玉米中报道。甜玉米籽粒中类胡萝卜素含量较高,最高可达39.68 mg/g(以干质量计),但品种间存在差异,主要组分为玉米黄质、叶黄素和α-隐黄质;糯玉米籽粒中类胡萝卜素含量较低(2.10~3.69 mg/g),主要组分为玉米黄质、新黄质和花药黄质。鲜食甜、糯玉米品种类胡萝卜素种类和含量存在明显不同。
In order to identify the characteristics of carotenoids in fresh corns from different cultivars, high performance liquid chromatography(HPLC)coupled with diode array detection(DAD) and atmospheric-pressure chemical ionizationmass spectroscopy(APCI-MS) were applied to identify and quantify the major carotenoids of fresh sweet and waxy corn grains from five cultivars. A total of 14 and 15 carotenoids were identified from sweet corn grains ‘Jingtian No.3' and ‘Jingtian No.5', respectively, while only a few carotenoids were detected in waxy corn grains ‘Suyunuo No.11', ‘Jingtianzihuanuo No.2' and ‘Jingnuo No.8'. Neochrome, antheraxanthin and 13-cis-lutein-5,6-epoxide were first reported in fresh corn. The content of carotenoid in sweet corn was highest, reaching 39.68 mg/g dry weight(DW), but there were differences in different varieties. The major carotenoids were zeaxanthin, lutein and α-cryptoxanthin. The content(about 2.10–3.69 mg/g DW) of carotennoid in waxy corn was lower. The major carotenoids were zeaxanthin, neoxanthin and antheraxanthin. Therefore, it might be concluded that the contents and chemical composition of carotenoids were extremely different among different cultivars of fresh sweet and waxy corns.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第18期108-113,共6页
Food Science
基金
江苏省农业科技自主创新资金项目(CX(14)2055)