高效液相色谱法对蓝莓果实中8个有机酸含量的测定

Determination of Eight Organic Acids in Blueberries by HPLC

建立同时测定蓝莓果实中8个有机酸类成分含量的高效液相色谱法,并利用该方法测定26个不同品种蓝莓果实中的有机酸含量。结果表明,所测品种中塞拉的有机酸含量最高,达25.135 26 mg/g鲜果,最低的为克瑞顿,仅含2.368 31 mg/g鲜果。该方法简便、快速、准确,具有良好的重复性和回收率,可作为蓝莓果实中有机酸含量的测定方法。

A high performance liquid chromatography（HPLC） method was established for the simultaneous determination of 8 organic acids in blueberry fruit extract. This method was then used to determine the contents of organic acids in blueberries from 26 cultivars. Among the blueberry cultivars tested, the content of total organic acids（25.135 26 mg/g fresh fruit） was highest in Sierra and lowest in Croaton（only 2.368 31 mg/g fresh fruit）. This method is simple, reproducible, accurate, reliable and useful for the analysis of organic acids in blueberries.