光动力治疗中氧分压和微弱荧光监测及成像系统的研究

Research on the pO_2 and Weak Fluorescence Monitoring and Imaging System in Photodynamic Therapy

针对深部肿瘤光动力治疗(PDT)过程中关键参数的采集,我们开发了一套实时在体的氧分压和微弱荧光监测及成像系统。该系统利用普林斯顿高灵敏度CCD采集微弱荧光图像,利用海洋光学氧传感器Neo Fox监测氧含量,采用Lab VIEW开发上位机控制程序,将荧光图像、氧分压、荧光强度数据、结果的显示、分析与存储等功能结合在一起,实现了光动力治疗过程中实时多参数监测与成像。经光动力治疗反应荧光实验,验证了系统的有效性,为后续PDT疗效评估研究工作奠定了基础。

Since the key multiple parameters need to be monitored during the photodynamic therapy（ PDT） in deep tumor,we developed a pO2 and weak fluorescence monitoring and imaging system. The Princeton Instrument scientific- grade CCD was used as the fluorescence imaging detector,NeoFox of Ocean Optics as O2 sensor and LabVIEW as programming platform. The function of the system included the fluorescence image measurement,the pO2 data acquisition,the fluorescence intensity detection,result display,data analyzing and recording simultaneously. The system realized the real- time multiple parameters measurement and analysis during PDT.The PDT chemical reaction experiments validate the sensitivity of system,which has a potential for assessment of PDT.