顶空气相色谱法测定交联透明质酸钠凝胶中异丙醇的残留量

Determination of the Residual Isopropanol in Cross- linked Sodium Hyaluronate by Gas Chromatography

建立了气相色谱法测定交联透明质酸钠凝胶中异丙醇残留量的方法。选用DB-624毛细管柱（30 mm×0.535 mm,3.0μm）,氢火焰离子化检测器（FID）,载气为氮气,流速为2.0 ml/min,柱温采用程序升温,初始温度为50℃,保持2 min,以10℃/min升温至160℃,后运行温度：50℃,保持1 min。异丙醇在浓度0.01083-0.8664 mg/ml的范围内,峰面积和浓度呈现良好的线性关系,相关系数0.9990;平均回收率为99.1%,相对标准偏差（RSD）为1.2%。该方法重现性好、灵敏度高、简单、准确。

A method for determination of resiual isopropanol in cross- linked sodium hyaluronate by GC chromatography was established. The method was programmed with Agilent DB- 624 capillary column（ 30 mm × 0. 535 mm,3. 0 μm） and FID detector. The carrier was nitrogen and the flow rate was 2. 0 ml / min,the oven introduced sequential increasing of temperature programming. The initial column temperature was 50℃ and maintained it for 2 min,then raised it to 160℃ at a rate of 10℃ / min,after running temperature to50℃,maintained it for 1 min. The calibration showed a good calibration linearity with the range of 0. 01083 - 0. 8664 mg / ml for isopropanol and the correlation coefficient was 0. 9990. The average recoveries were in the range of 99. 1%. The relative standard deviations（ RSD） was 1. 2%. The method has good repeatability and high sensitivity and the method is simple and accurate.