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海参皂苷单体Echinoside A在大鼠体内的消化吸收特性初步研究 被引量:1

The preliminary study on the digestion and absorption characteristics of sea cucumber saponins Echinoside A in rats
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摘要 目的以大鼠为动物模型研究了海参皂苷单体EchinosideA(EA)在体内的消化吸收特性。方法Wistar大鼠适应饲养1周后,按体质量分为10组,每组3只。禁食不禁水12h后,给大鼠分别按照30mg/kg·BW灌胃配置好的海参皂苷EA溶液1mL,分别在灌胃后0、0.5、1、2、3、4、7、9、11、24h取血,建立并优化血清中海参皂苷EA的HPLC-MS检测方法,检测不同时间节点大鼠血中EA含量。结果实验结果显示,海参皂苷单体EA液质检测的定量限(LOQ)为2.12ng,在0.3~20,ug/mL的浓度范围内线性良好(R。=0.9946)。海参皂苷EA灌胃后在血中迅速出现,在3h出现最高峰值,浓度达到0.83/μg/mL,之后在7h出现另1个较小峰,9h后恢复至4h水平(约0.24/μg/mL),直至24h。结论本文建立了血清中海参皂苷EA的液相色谱-质谱联用检测方法,发现海参皂苷单体EA可经消化道吸收,该结果为海参皂苷在保健食品及药物中的应用提供了科学依据。 Objective To study the digestion and absorption characteristics of sea cucumber saponins Echi- noside A (EA) in rats. Methods Wistar rats were divided into 10 groups (n=3) according to the body weight. After acclimating for 1 week, the animals were fasted for 12 hours, and oral administration of 1 mL sea cucumber saponins EA solution at the dose of 30 mg/kg BW. The blood was collected in 0, 0. 5, 1, 2, 3, 4, 7, 9, 11, 24 h for the serum determination of EA. The HPLC-MS method of serum EA determination was established and optimized. Results The limit of quantification (LOQ) of EA detected by HPLC-MS was 2. 12 ng, and it showed a good linearity in the concentration range from 0. 3 to 20 μg/mL (R2 =0. 9946). Sea cucumber saponins EA was appeared rapidly after oral administration, the highest peak concentration reached 0. 83 μg/mL at 3 h, and another smaller peak showed at 7 h, then returned to the level of 4 h (about 0.24 μg/mL) at 9 h and stayed the level to 24 h. Conclusion This pa- per established HPLC-MS method of sea cucumber saponins EA in serum, and found that the sea cu- cumber saponins EA could be absorbed by gastrointestinal, the results provided scientific evidence for the application of sea cucumber saponins in marine functional food and drugs.
出处 《中国海洋药物》 CAS CSCD 2015年第5期41-46,共6页 Chinese Journal of Marine Drugs
基金 国家自然科学基金项目(U1406402) 新世纪优秀人才项目(NCET-13-0534) "泰山学者"建设工程专项经费资助
关键词 海参皂苷 Echinoside A 消化吸收 HPLC-MS 保健食品 sea cucumber saponins Echinoside A digestion and absorption HPLC-MS functional food
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参考文献11

  • 1阮伟达,苏永昌,吴成业.海参皂苷的研究现状[J].福建水产,2011,33(2):74-78. 被引量:13
  • 2Van Dyck Séverine,Gerbaux Pascal,Flammang Patrick.Qualitative and quantitative saponin contents in five sea cucumbers from the Indian ocean[J].Marine Drugs,2010,81:173-89.
  • 3薛长湖,于林芳,董平,徐杰,李兆杰,薛勇.青岛仿刺参中3种海参皂苷的分离纯化及结构鉴定[J].中国海洋大学学报(自然科学版),2010,40(8):60-65. 被引量:11
  • 4Qing Fang Xu,Xiao Ling Fang,Dao Feng Chen.Pharmacokinetics and bioavailability of ginsenoside Rb1and Rg1 from Panax notoginsengin rats[J].Journal of Ethnopharmacology,2003,84:187-192.
  • 5温平,胡瑞君.植物中皂苷类成分的检测技术进展[J].价值工程,2010,29(20):113-114. 被引量:3
  • 6Hye Young Ji,Hye Won Lee,Hae Kyoung Kim,et al.Simultaneous determination of ginsenoside Rb 1 and Rg1 in human plasma by liquid chromatography-mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2004,351:207-212.
  • 7Peng Dacheng,Wang Huashan,Qu Chenling,et al.Ginsenoside Re:Its chemistry,metabolism and pharmacokinetics.[J].Chinese Medicine,2012,7:2.
  • 8Kyung-Mi Joo,Ji-Hae Lee,Hee-Young Jeon,et al.Pharmacokinetic study of ginsenoside Re with pure ginsenoside Re and ginseng berry extracts in mouse using ultra performance liquid chromatography/mass spectrometric method[J].Journal of Pharmaceutical and Biomedical Analysis.2010,51:278-283.
  • 9Huai-You Wang,Hai-Ying Hua,Xing-Yan Liu,et al.In vitro biotransformation of red ginseng extract by human intestinal microflora:Metabolites identification and metabolic profile elucidation using LC-Q-TOF/MS[J].Journal of Pharmaceutical and Biomedical Analysis,2014,98:296-306.
  • 10Hu Xiaoqian,Li Zhaojie,Xue Yong,et al.Dietary saponins of sea cucumber ameliorate obesity,hepatic steatosis,and glucose intolerance in high-fat diet-fed mice[J].Journal of Medicinal Food,2012,1510:909-916.

二级参考文献53

  • 1巫军,易杨华,吴厚铭,贺全山,邹峥嵘,张诗龙.黑乳海参中两个新的四环三萜化合物[J].中国天然药物,2005,3(5):276-279. 被引量:10
  • 2韩玉谦,冯晓梅,管华诗.海参皂甙的研究进展[J].天然产物研究与开发,2005,17(5):669-672. 被引量:26
  • 3丛日山,袁文鹏,樊廷俊,郭瑞超,宣昭,张春阳,蔡慧坤.仿刺参水溶性海参皂苷的分离制备及抗真菌活性的研究[J].中国海洋大学学报(自然科学版),2006,36(6):959-964. 被引量:26
  • 4翁怡毅,易杨华,李玲,张诗龙,潘敏翔,刘宝姝,路小娟,薛梅.沙海参中的三个海参皂苷[J].中国天然药物,2007,5(2):96-100. 被引量:8
  • 5Hye Young Ji,Hye Won Lee,Hae Kyoung Kim,et al.Simultaneous determination of ginsenoside Rb1 and Rg1 in human plasma by liquid chromatography-mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2004,35(1):207.
  • 6Nah JJ.Preparation of monoelonal antibody against ginsenoside Rf and its enzyme immunoassay[J].Biol Pharm Bull,2000,23(5):523.
  • 7Yoon S R,Nah JJ,Kim S K,et al.Detemination of ginsenoside Rf and Rg2 from Panax ginseng using enzyme immunoassay[J].Chen Pharm Bull,1998,46(7):1144.
  • 8Nigrelli R F.The effects of holothurin on fish,and mice with sarcoma 180[J].Zoologica,1952,37:89-90.
  • 9Yamanouchi T.On the poisonous substance contained in holothurians[J].Publ Seto Mar Biol Lab,1955,4:183-202.
  • 10Nguyen H D,Nguyen V T,Phan V K,et al.Two new tritenpene glycosides from the Vietnamese sea cucumber Holothuria scabra.[J].Arch Pharm Res,2007,30:1387-1391.

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