海参皂苷单体Echinoside A在大鼠体内的消化吸收特性初步研究

The preliminary study on the digestion and absorption characteristics of sea cucumber saponins Echinoside A in rats

目的以大鼠为动物模型研究了海参皂苷单体EchinosideA（EA）在体内的消化吸收特性。方法Wistar大鼠适应饲养1周后，按体质量分为10组，每组3只。禁食不禁水12h后，给大鼠分别按照30mg／kg·BW灌胃配置好的海参皂苷EA溶液1mL，分别在灌胃后0、0．5、1、2、3、4、7、9、11、24h取血，建立并优化血清中海参皂苷EA的HPLC-MS检测方法，检测不同时间节点大鼠血中EA含量。结果实验结果显示，海参皂苷单体EA液质检测的定量限（LOQ）为2．12ng，在0．3～20,ug／mL的浓度范围内线性良好（R。=0．9946）。海参皂苷EA灌胃后在血中迅速出现，在3h出现最高峰值，浓度达到0．83／μg／mL，之后在7h出现另1个较小峰，9h后恢复至4h水平（约0．24／μg／mL），直至24h。结论本文建立了血清中海参皂苷EA的液相色谱-质谱联用检测方法，发现海参皂苷单体EA可经消化道吸收，该结果为海参皂苷在保健食品及药物中的应用提供了科学依据。

Objective To study the digestion and absorption characteristics of sea cucumber saponins Echi- noside A （EA） in rats. Methods Wistar rats were divided into 10 groups （n=3） according to the body weight. After acclimating for 1 week, the animals were fasted for 12 hours, and oral administration of 1 mL sea cucumber saponins EA solution at the dose of 30 mg/kg BW. The blood was collected in 0, 0. 5, 1, 2, 3, 4, 7, 9, 11, 24 h for the serum determination of EA. The HPLC-MS method of serum EA determination was established and optimized. Results The limit of quantification （LOQ） of EA detected by HPLC-MS was 2. 12 ng, and it showed a good linearity in the concentration range from 0. 3 to 20 μg/mL （R2 =0. 9946）. Sea cucumber saponins EA was appeared rapidly after oral administration, the highest peak concentration reached 0. 83 μg/mL at 3 h, and another smaller peak showed at 7 h, then returned to the level of 4 h （about 0.24 μg/mL） at 9 h and stayed the level to 24 h. Conclusion This pa- per established HPLC-MS method of sea cucumber saponins EA in serum, and found that the sea cu- cumber saponins EA could be absorbed by gastrointestinal, the results provided scientific evidence for the application of sea cucumber saponins in marine functional food and drugs.