摘要
[目的]了解兔源产ESBL大肠杆菌耐药特点,为掌握其造成的耐药性危害提供数据参考。[方法]2015年3-4月从青岛4个养兔场采集118份兔粪便棉拭子,进行大肠杆菌分离,并检测对16种抗生素的耐药谱,及对多重耐药调控基因SoxS携带率。[结果]从118份兔粪便棉拭子分离获得68株大肠杆菌,确证筛选出47株产ESBL大肠杆菌,占兔源大肠杆菌的69.1%。药敏结果显示所有产ESBL大肠杆菌菌株耐药性较强且呈现多重耐药,仅对碳青霉烯类药物敏感率100%。兔源产ESBL大肠杆菌多重耐药调控基SoxS携带率为100%,且不同动物源性产ESBL大肠杆菌的多重耐药调控基因同源关系较近。[结论]青岛地区兔源产ESBL大肠杆菌分离率高且耐药谱广,多重耐药调控基因SoxS携带率高,主要涉及到细菌主动外排泵出机制,使得大肠杆菌对抗生素的敏感性中起到了至关重要的作用,本研究对抗生素在临床中的应用起指导意义。
[Objective] To understand the antimicrobial characteristics of ESBL-producing E. coli strains from rabbits and provide important data for reference to know their antimicrobial hazard. [Methods] 118 rabbit fecal samples were taken from 4 rabbit farms in Qingdao region for E. coli isolation and anti-microbial identification. [Results] 68 E. coil strains were isolated from the 118 rabbit fecal samples with 47 ESBL-producing E. coli strains, all of which were multi-drug resistant but sensitive to carbapenems. Polymerase chain reaction and cloning of the SoxS gene demonstrated that the multi-drug resistant SoxS gene carrying rate ofESBL-producing E. coli strains from rabbit was 100%. [Conclusion] The isolation rate of ESBL-producing E. coli strains from Qingdao region was high with wide drug resistance spectrum. High rate of multi-drug resistant SoxS gene was mainly involved in the bacterial efflux pump mechanism, playing key role in sensitivity ofE. coli to antibiotics. The study provided guidance for clinical application of antibiotics.
出处
《中国动物检疫》
CAS
2015年第10期28-31,共4页
China Animal Health Inspection
