沙疗下不同月龄兔膝骨关节炎股骨的骨质骨量及生物力学性能变化

Change of Volume and Mechanical Properties of Femoral Bone in Rabbits at Different Ages with Knee Osteoarthritis under Sand-therapy

研究维医沙疗对不同月龄兔骨性关节炎(OA)股骨的骨质骨量变化及生物力学性能的影响。实验选用新西兰大白兔成熟期组16只,生长期组16只,对其右后股骨膝关节腔注射木瓜蛋白酶建OA模型,成熟期组随机分2组:对照组(不沙疗)8只,沙疗组8只;生长期组随机分2组:对照组(不沙疗)8只,沙疗组8只;采用CT扫描4次(建模前1d、建模第13、27、41d),MIMICS软件获取股骨模型、各骨质的骨体积和骨体积比的变化;最后进行股骨三点弯曲试验,获得弹性载荷和弹性挠度并计算截面惯性矩、最大弯曲正应力、弯曲弹性模量和结构刚度。实验结果表明:1成熟期兔股骨OA建模前1d与第13d相比较,松质骨量增多而皮质骨量减少(P<0.05);而各自相比对照组,沙疗组在沙疗作用下OA兔股骨松质骨量减少和皮质骨量增多(P<0.05)。2各自相比对照组,沙疗组OA兔股骨挠度和横截面惯性矩无统计学差异(P>0.05),而截面面积、最大弯曲正应力、最大载荷、弹性模量和结构刚度变大(P<0.05)。本研究显示:1维医沙疗对不同月龄兔OA股骨的各骨质骨量变化都有良性影响。2维医沙疗能改善不同月龄兔OA股骨的力学性能。

The present paper is aimed to study the effect of sand therapy with Uyghur medicine on biomeehanical properties and femoral bone volume of the femur of osteoarthritis （OA） rabbits at two different ages. In the experi- ments, we injected Papain through the joint space of right knees into the bodies of New Zealand rabbits （16 in the growing group, and 16 in the mature group）, and established an OA model. The 16 rabbits in the mature group were divided randomly into 2 sub-groups： 8 in control group （no sand therapy）, and 8 sand-therapy group. The 16 rabbits in the growing group were divided randomly into 2 groups as well： 8 in the control group （no sand therapy）, and 8 in the sand-therapy group. We carried out CT .scanning four times （1 day before, 13th, 27th and 41st days after the es- tablishment of the model, respectively）. After importing the CT data to MIMICS, the different volumes of each scle- rotin were recorded and change of the percentage of each selerotins in total femur bone volume were analyzed. Finally the rabbit femurs were given three-point bending test, the elastic load and the elastic deflection were obtained and theinertia of the section, the maximum bending stresses, the bending modulus of elasticity and the structural rigidity were calculated. The experimental results showed that ① Compared with 1 day before and 13th day after establish- ment of model at maturity, the cancellous bone volume increased and cortical bone volume decreased （P〈0.05）, but compared with those in the control group, the cancellous bone volume of femurs decrease and cortical bone volume increased under sand-therapy （P〈0.05）. Compared with those in the control group, there were no significant chan ges in the deflection and cross-sectional moment of inertia in the sand-therapy group （P〉0.05）, but the maximum bending normal stress, maximum load, elastic modulus, and structural stiffness （P〈0.05） in the sand therapy group were larger than those in the control group. The study showed that sand-therapy in Uyghur medicine has be- nign effect on bone volume of the OA rabbit femur in two ages and the mechanical properties of the OA rabbit femur at the two ages induced by sand therapy in Uyghur medicine have been improved.