下丘脑-小脑神经通路在大鼠躯体-内脏反应整合中的作用

The role of hypothalamocerebellar histaminergic projections in somatic-visceral integration of rats

目的探索SD大鼠下丘脑-小脑神经通路在躯体-内脏反应整合过程中发挥的作用。方法将120只SD大鼠随机分为6组;分别在大鼠小脑深部核团定点微量注射生理盐水(A组),组胺H1受体阻断剂triprolidine(B组),组胺H1受体激动剂2-PyEA(C组),组胺H2受体阻断剂ranitidine(D组),组胺H2受体激动剂dimaprit(E组),以及神经递质组胺(F组)。以A组为对照,观察下丘脑-小脑组胺能神经纤维信号被阻断或强化后,大鼠躯体-内脏反应整合能力的变化。结果与A组相比,B组和C组无明显效应,而D组大鼠躯体-内脏反应整合被明显阻断,而E组和F组大鼠躯体-内脏反应整合被明显强化。结论小脑组胺能传入纤维参与了机体躯体-内脏反应整合的调控,并且是通过H2受体途径发挥效应的。

Objective To research the role of hypothalamocerebellar histaminergic projections in somatic-visceral integration of rats.Methods A total of 120 SD rats were randomly divided to 6groups：group A-F;which separately microinjected saline（group A）,triprolidine（selective antagonist of H1 receptor,group B）,2-PyEA（selective agonist of H1 receptor,group C）,ranitidine（selective antagonist of H2 receptor,group D）,dimaprit（selective agonist of H2 receptor,group E）and histamine（group F）to the deep nuclei of rats.The abilities of rats somatic-visceral integration were observed when hypothalamocerebellar histaminergic projections were obstructed or enhanced compared to group A.Results The somatic-visceral integration of rats were obstructed obviously in group D and enhanced in group E group F compared to group A,while group B and group C had no differences compared to group A.Conclusion Hypothalamocerebellar histaminergic projections play a key role in somatic-visceral integration through the way of H2 receptors.