尿激酶型纤溶酶原激活物在子宫内膜异位症中的表达及意义

The significance and expression of urokinase plasmino-gen activator in the endometriosis

目的研究尿激酶型纤溶酶原激活物(uPA)在子宫内膜异位症(EMs)组织中的表达及意义。方法分别采用免疫组织化学SP法及RT-PCR法检测正常子宫内膜组织30例,EMs的在位内膜35例,EMs的异位内膜35例,uPA蛋白及mRNA的表达。结果 uPA蛋白主要定位于子宫内膜腺上皮细胞与间质细胞的细胞质中,极少数表达于血管内皮细胞中,在EMs异位内膜中的表达强于在位内膜、正常内膜,在EMs在位内膜中的表达强于正常内膜(P<0.05)。uPA蛋白在3组内膜的增生期与分泌期中的表达差异无统计学意义(P>0.05)。uPA mRNA在EMs患者异位内膜中的相对表达量高于在位内膜和正常内膜,在内异症在位内膜中的表达高于正常内膜(P<0.05)。结论 uPA可能在EMs异位内膜的黏附、生长等方面起一定作用。

Objective To investigate the significance and expression of uPA in the endometriosis.Methods Expressions of uPA in 30 cases of normal endometrium,35 cases of eutopic endometrium were detected by the immunohistochemical SP and RTPCR methods.Results First,in three groups,the expression of uPA protein was mainly in the cytoplasm of endometrial glandular epithelial cells and stromal cells,few was expressed in vascular endothelial cells,which the expression of uPA protein in ectopic endometrium of EMs was higher than eutopic and normal endometrium,with significantly differences among 3groups（P〈0.05）.Second,in three groups,there was no differences between the expression of uPA in proliferative and progestational stage（P〈0.05）.Third,the relative expression of uPA mRNA in ectopic endometrium of EMs was higher than eutopic and normal endometrium,with a significant difference among 3groups（P〈0.05）.Conclusion uPA may play an important role in the pathogenisis of EMs.