黄连素对K562细胞增殖和凋亡的影响

Effect of Berberine on proliferation and apoptosis of K562cells

目的:探讨黄连素对人慢性髓系白血病细胞K562的作用及其机制。方法:将对数生长期的K562细胞随机分成对照组和黄连素组。对照组细胞常规培养,黄连素组细胞培养体系中加入黄连素(40μmol/L)。各组细胞培养24h后,利用MTT检测细胞增殖;RT-PCR检测细胞Bax和Bcl-2基因表达水平;Western blotting检测细胞JAK2,STAT3,p-JAK2,p-STAT3,Bax和Bcl-2蛋白的变化。结果:与对照组比较,黄连素组K562细胞增殖率明显下降(P<0.05)。RT-PCR检测显示Bax mRNA表达水平明显增加,而Bcl-2mRNA表达水平明显减少。Western blotting检测显示,黄连素组与对照组比较,K562细胞JAK2、STAT3蛋白表达无明显变化(P>0.05),但p-JAK2、p-STAT3和Bcl-2蛋白表达水平明显降低,而Bax蛋白表达水平明显增加(P<0.05)。结论:黄连素能诱导K562细胞增殖抑制和凋亡,其机制可能是通过抑制JAK2/STAT3信号传导通路实现的。

Objective： To explore the effect and mechanisms of the Berberine on the human leukemia cell line K562. Method.. The K562 cells at logarithmic growth phase were randomly divided into control and Berberine groups. The cells in the control group were normally treated and cells in Berberine group were incubated with Berberine （40 μmol/L）. MTT and Annexin-V were used to examine the proliferation and apoptosis of the cells in the two groups after incubation for 24 h respectively. The mRNA levels of Bax and Bcl-2 were detected by RT-PCR. The expression levels of JAK2, STAT3, p-JAK2, p-STAT3 ,Bax and Bcl-2 protein were detected by Western blot- ting. Result：Compared with the control group, the proliferation inhibition, apoptosis and the Bax mRNA level of K562 cells in Berberine group were significantly increased with the decrease of Bcl-2 mRNA level, the expression of p-JAK2, p-STAT3 and Bcl-2 protein（P〈0.05）. Moreover, there was no difference on the expression level of JAK2 and STAT3 protein between two groups （P〉0.05）. Conclusion.. Berberine could induce the growth inhibition and apoptosis of K562 cells and these effect may be attributed to suppressing JAK2/ STAT3 signal transduction path- way activation.