正交提取法优选刺头复叶耳蕨总黄酮的提取工艺

Optimization of Extraction Process for Total Flavonoids of Arachniodes Exilis Ching

目的研究超声波提取法从刺头复叶耳蕨中提取总黄酮的最佳工艺。方法以总黄酮提取率为评价指标,采用单因素试验及正交试验对超声波提取法的过程参数进行优化。结果所得最佳提取工艺:溶剂为60%乙醇,料液比为1∶20,超声波辅助提取3次,每次提取30 min,总黄酮的提取率可达到16.18 mg/g(总黄酮量/生药量)。结论该提取工艺的优化结果合理,超声波提取法得到刺头复叶耳蕨总黄酮可行。

Objective To study the optimum ultrasonic extraction process for total flavonoids from Amehniodes exilis ehing. Method With total flavonoids content as index, the extraction of total flavonoids from Araehniodes exilis thing by orthogonal experiment in the optimum ultrasonic extraction process was optimized. Result The optimum ultrasonic extraction process was using 60% ethanol, 1 ： 20 solid - liquid ratio, ultrasonic extraction 30 rain with three times. Under these conditions, the yield of total flavonoid extract were 16. 18 mg/g （total flavonoid/crude drug）. Conclusion The optimized ultrasonic extraction process was reasonable and feasible with high yield.