偶氮染料与蛋白质相互作用的分子光谱法研究

Study on the Molecular Spectroscopy of the Interaction between Azo Dyes and Proteins

采用荧光偏振技术,运用荧光光谱法、紫外光谱法和三维荧光光谱法研究了在p H=7的PBS缓冲介质中,不同浓度的丽春红S(Ponceau S)和一定量牛血清白蛋白(bovine serum albumin,BSA,以下简称BSA)的光谱作用。荧光光谱表明随着染料浓度的增加,荧光偏振强度(FP)呈下降的趋势,其关系式为:F0/F=0.0011C+1.1435,R=0.9938,说明丽春红S与BSA相互作用产生了荧光猝灭;紫外光谱表明,随着染料浓度的增加,吸光度呈增大的趋势。

The spectral effects of S（S Ponceau）with different concentration and serum bovine（BSA）with certainamount in the buffer medium of PBS with p H=7 were studied by fluorescence polarization technique,fluorescencespectroscopy,UV spectroscopy and three-dimensional fluorescence spectroscopy. Fluorescence spectra showed thatthe intensity of fluorescence polarization（FP）decreased with the increase of dye concentration,and the relationshipwas： =0.0011C＋1.1435 R=0.9938,F0/F. That meant fluorescence quenching was produced by the interaction of Pon-ceau S and bovine serum albumin（BSA）;UV spectra showed that with the increase of the dye concentration,absor-bance had a roughly increasing trend.