川芎嗪在大鼠肠吸收机理及当归提取液对其吸收的影响

The Absorption Mechanism of Tetramethylpyrazine in Rat and the Impact of Angelica sinensis Extraction

研究川芎嗪在大鼠肠吸收动力学,考察不同质量浓度川芎嗪及不同体积比的当归提取液混合物对其吸收速率及相关参数的影响.运用大鼠在体单向肠灌流法测定,重量法校正系统误差,HPLC测定吸收前后药物质量浓度的变化,考察其吸收动力学及当归提取液对其吸收的影响.结果表明,不同质量浓度(10,20,40 mg/L)川芎嗪的大鼠肠吸收速率常数分别为(0.006 39±0.052 3),(0.006 51±0.019 1),(0.006 03±0.073 7)min-1,无显著性差异;川芎嗪标准溶液与当归提取液按体积比为1∶2,1∶1,2∶1混匀时,川芎嗪的吸收速率常数分别为(0.012 32±0.106 4),(0.010 24±0.086 1),(0.006 195±0.079 9)min-1,结果显示,川芎嗪的吸收速率常数与当归提取液占混合液的体积分数呈现正相关.川芎嗪在大鼠肠道为被动吸收过程,当归提取液对其有一定的促吸收作用,且随着当归提取液量的增加而增大.

The absorption kinetics of tetramethylpyrazine（ TMP） in small intestine of rats and the concentration influence of TMP and Angelica sinensis extraction on it were investigated. The effect of TMP＇s absorption in small intestine of rats was studied by using the in situ rats single-pass intestinal perfusion model and HPLC. And the impact of Angelica sinensis extraction on the absorption of TMP was obtained. The results showed that the absorptive rate constants（ k） of TMP at the concentration of 10,20,40 mg / L were（ 0. 006 39 ± 0. 052 3）,（ 0. 006 51 ± 0. 019 1）,（ 0. 006 03 ± 0. 073 7） min- 1,while adding different quantity of Angelica sinensis extraction to the TMP, the k of TMP were（ 0. 012 32 ± 0. 106 4）,（ 0. 010 24 ± 0. 086 1）,（ 0. 006 195 ± 0. 079 9） min- 1. The absorption mechanism of TMP was passive diffussion. Angelica sinensis extraction could promote the absorption of TMP in small intestine of rats,the concentration of Angelica sinensis extraction had some effect on the absorption of TMP.