阻塞性黄疸患者结肠菌群失衡的实验研究

Experimental Study of Colonic Microflora Imbalance in Patients with Obstructive Jaundice

目的探讨阻塞性黄疸患者与健康人肠道菌群结构的变化特征。方法选取笔者所在医院2013年2月至2014年8月期间连续性20例阻塞性黄疸患者及同期10例健康体检人员(正常对照组)作为研究对象,留取阻塞性黄疸患者入院后晨起第1次粪便,提取粪便总的微生物DNA,经PCR扩增后行变性梯度凝胶电泳(DGGE),动态观察菌群结构变化,分析凝胶条带,对菌群结构进行相似性、多样性和主成分分析。结果阻塞性黄疸患者结肠微生物结构与正常对照组的结肠微生物结构之间存在明显差异;阻塞性黄疸患者肠道优势菌群数量明显低于正常对照组,随着梗阻时间的延长及梗阻程度的加重,其肠道优势菌群在数量、丰富度及多样性上呈逐渐下降趋势(P<0.05)。结论阻塞性黄疸患者结肠菌群结构与健康人的结肠菌群结构存在明显差异。

Objective To explore the variation of the structure of the intestinal flora between healthy people and patients with obstructive jaundice perioperatively. Methods From February 2013 to August 2014, 20 patients with obstructive jaundice and 10 healthy persons （normal control group） in our hospitol were selected as the research object. The first stool specimens of the research object after admission were obtained and the total fecal bacteria DNA were extracted. After polymerase chain reaction amplification, the changes in the structure of bacterial flora were dynamic observed by using denaturing gradient gel electrophoresis （DGGE）, and the gel bands were analyzed by using Quantity One software. The similarity and diversity of flora structure, and principal component analysis （PCA） were analyzed. Results Significant differences of colonic microflora were found between patients with obstructive jaundice and healthy people; advantage intestinal flora in obstructive jaundice patients was significant lower than the normal control group. With the extension of time and degree of obstruction aggravated, a descending trend was found in number, abundance, and diversity of the intestinal microflora （P〈0.05）. Conclusion There is significant differences in the structure of colon bacteria in patients with obstructive jaundice and healthy persons.