辽阳市2011—2013年HIV抗体确证结果分析

Analysis on confirmation results of HIV antibody test in Liaoyang city,2011-2013

目的了解辽阳市HIV抗体筛查、确证检测情况,为辽阳市艾滋病预防检测工作提供更合理的指导依据。方法收集2011—2013年辽阳市HIV筛查阳性样本,采用蛋白印迹(WB)法进行检测,c2统计分析。结果辽阳市2011—2013年128份HIV抗体筛查阳性的样本,WB确证85份为阳性,阳性率为66.41%;15份为不确定,占11.72%;28份为阴性,占21.88%。3者间S/CO分布差异有统计学意义(χ2=111.794,P<0.05)。且S/CO值>6时与确证阳性符合率达95.3%(81/85),确证阳性出现全带型为42份,占49.4%。结论 HIV抗体筛查实验存在一定数量的假阳性,即使S/CO值>6时也不能完全代替确证实验。不确定样本,随访同时须结合流行病学资料及P24抗原、病毒载量检测结果以便做出正确判断。

Objective To examine the status of human immunodeficiency virus （HIV）antibody screening and confirmation test in Liaoyang city and to provide evidences for improving HIV infection detection in the city. Methods Western blot（WB）assay was adopted in the confirmation test according the requirement of National Technical Manual for HIV testing;chi-square test was used in data analysis. Results Of the 128 HIV antibody positive specimens during the period of 2011 - 2013 in the city, 85 （66.41% ） were positive, 28 （21.88 % ） were negative, and 15 （ 11.72 % ） were indeterminate based on the results of WB confirmation assay and there was a statistically significant difference in the distribution of standard deviation/cut off value（S/CO） among the screening results with the three types of confirmation outcome （χ^2 = 111. 794, P 〈 0. 05 ） ;for the specimens with the S/CO value of 〉 6 in the screening test, the WB confirmation positive rate was 95.3% （ 81/85 ） and 49.4% （42/85） of the specimens showed full pattern in WB band type. Conclusion There are false positive results in HIV antibody screening, even for the specimens with the S/CO value of 〉 6 and confirmation test is needed.