HPLC法测定夜关门药材中槲皮素的含量

Determination of quercetin in Crneate Lespedeza by HPLC

目的：建立夜关门药材中槲皮素含量的测定方法。方法：采用HPLC法,色谱柱：Agilent HC-C18（150mm×4.6 mm,5μm）;流动相：甲醇-0.3%磷酸水溶液（55∶45）;检测波长370nm;流速：1mL·min-1。结果：槲皮素在1.84μg·mL-1~18.4μg·mL-1范围内与峰面积呈良好线性关系（r=0.9992）;平均回收率为97.7%,RSD=1.99%。结论：建立的方法灵敏、准确、重现性好,可有效控制夜关门药材的质量。

Objective：To establish the method for determination of quercetin in Crneate Lespedeza.Methods：The content of quercetin was determined by HPLC.The column was Agilent HC-C18column（150mm×4.6mm,5μm）.The mobile phase was Methanol and 0.3% phosphoric acid solution（55∶45）.The detection wavelength was 370 nm.The flow rate was 1mL·min-1.Results：Linearity range of quercetin was 1.84μg·mL-1~18.4μg·mL-1（r=0.9992）.The average recovery was 97.7% and RSD was 1.99%.Conclusion：The methods are proved to be simple,accurate and reproducible.It can effectively control the quality of Crneate Lespedeza.