磷氧氮丙啶不育剂对雄性红耳龟生殖毒性研究

Reproductive Toxicity Research of MAPO on Male Trachemys scripta elegans

目的研究磷氧氮丙啶(MAPO)对红耳龟Trachemys scripta elegans睾丸形态学及组织病理学的影响,从控制繁殖的途径初步探讨外来物种红耳龟的防治。方法选取160只健康成体雄性红耳龟,随机平均分为4组,设置MAPO相应浓度梯度,对照组和3个试验组(0.1 g·L-1、3 g·L-1、10 g·L-1),腹腔注射15 d。结果低、中剂量组平均摄食量与对照组相比变化不明显(P>0.05),高剂量组平均摄食量、睾丸体积及脏器系数显著减小(P<0.05);组织病理学观察显示,各处理组睾丸均有不同程度的损伤,中、高剂量组曲细精管萎缩,各级生精细胞几乎全部退化消失,生精上皮厚度极显著低于对照组(P<0.01);MAPO对血清和睾丸中睾酮含量无影响。结论MAPO可通过作用各级生精细胞的发育,导致不育,可进一步开发为红耳龟不育防控药物,应用于生产实践。

Objective To investigate the testicular morphology and histopathology effect of MAPO on male Trachemys scripta elegans,we preliminarily investigated the way to prevent T. scripta elegans by breeding control. Methods A total of160 adult health male T. scripta elegans were randomly divided into three experiment groups and one control group. The turtles in three experiment groups were treated with normal saline at the concentration of 0. 1 g·L- 1,3 g·L- 1,10 g·L- 1MAPO by intraperitoneal injection for 15 days,respectively; the turtles in control group were treated with normal saline for15 days. Results Compared with the control group,the food consumption of low-dose group and middle-dose group were not significantly different（ P〈 0. 05）; the volume and organ coefficient of testis and the food intake in high-does group declined very obviously（ P 〈0. 01）. According to pathologic histological observation,the testis of all the MAPO treated male turtles showed a certain degree of damage,and the seminiferous of the middle-does group and high-does group were atrophied. The majority of germ cells were necrotic and the thickness of seminiferous epithelium diminished very significantly compared with the control group（ P〈 0. 01）. MAPO had no effect on testosterone in serum and testis. Conclusions MAPO can cause dysgenesis of T. scripta elegans by blocking the development of germ cell and can be used to control its population size.