溶栓胶囊对脑缺血缺氧耐受和脑储备能力影响的实验研究

Effect of Rong-Shuan Capsule on Rodent Tolerance against Cerebral Ischemia, Hypoxia and Cerebral Reserve Capacity

目的:观察具有活血化瘀功效的溶栓胶囊对脑缺血缺氧耐受和脑储备能力影响。方法:采用左侧颈总动脉结扎并通入氧气和氮气(8?92)15 min的方法建立C57 BL/6小鼠急性脑缺血缺氧模型,造模后10 min十二指肠给药,给予不同剂量的溶栓胶囊(100、200、400 mg·kg-1)或生理盐水,评价局部脑血流量变化以及24 h后的神经行为学改变。SD大鼠给予不同剂量的溶栓胶囊(75、150、300 mg·kg-1)或生理盐水,观察溶栓胶囊对高分子右旋糖苷诱导的大鼠微循环障碍的改善作用。结果:与假手术组比较,模型组小鼠脑血流量明显下降,脑缺血范围增加,脑缺氧后行为学评分明显升高(P<0.05或P<0.01);同时,模型组大鼠在注射高分子右旋糖苷后软脑膜血管出现微循环障碍,10、20、30 min 3个时间点大鼠软脑膜微循环血液流速明显减慢。与模型组比较,溶栓胶囊(400 mg·kg-1)可以明显增加小鼠局部脑血流量,改善行为学障碍,减小脑缺血范围(P<0.05或P<0.01);溶栓胶囊还可改善高分子右旋糖苷诱导的大鼠软脑膜微血管不同时间点血液流速和流态(P<0.05或P<0.01)。结论:溶栓胶囊可通过增加脑缺血缺氧耐受和脑储备能力而达到保护缺血缺氧神经组织的作用,促进神经功能的恢复。

This study was aimed to observe the effect ofRong-Shuan （RS） capsule on rodent tolerance against cerebral ischemia, hypoxia and cerebral reserve capacity, which was related to promote blood circulation and remove blood stasis. Acute cerebral ischemia and anoxia models were established by permanent left carotid artery ligation on C57 BL/6 mice and hypoxia inhalation （O2：N2 = 8？92） for 15 min. Duodenal administration of RS capsule at different doses （100, 200 or 400 mg·kg-1） or saline were given 10 min after ischemia onset. The local brain blood circulation changes and neurobehavioral function were evaluated 24 h after ischemia onset. SD rats were given RS capsule at different doses （75, 150, 300 mg＆#183;kg-1） or saline. The effect of RS capsule on improvement of microcirculation disturbance induced by high molecular dextran was observed. The results showed that compared with the sham operation group, the brain blood circulation in the model group was significantly decreased; the cerebral infarction area increased; and the behavioral score after cerebral hypoxia was significantly increased （P〈 0.05, orP 〈 0.01）. Meanwhile, after the injection of high molecular dextran among rats in the model group, the cerebral leptomeninx microcirculation was obviously slowed down at 3 timepoints, which were 10, 20 and 30 min. Compared with the model group, RS capsule （400 mg＆#183;kg-1） can significantly increase the local blood circulation in the brain of mice, improve behavioral disturbance, reduce cerebral ischemia area （P〈 0.05, orP 〈 0.01）. RS capsule can also improve blood flow velocity and flow pattern in cerebral leptomeninx microcirculation disturbance induced by high molecular dextran at different timepoints （P 〈 0.05, orP 〈 0.01）. It was concluded that RS capsule can increase the tolerance against cerebral ischemia, hypoxia and cerebral reserve capacity in order to protect the neural tissues to promote neuronal recovery.