骨唾液酸蛋白在前列腺癌组织中的表达及其临床意义

Expression of bone sialoprotein in prostate cancer and its clinical significance

目的：探讨骨唾液酸蛋白（BSP）在前列腺癌组织中的表达及其意义。方法选取不同分级的前列腺癌组织（68例）和良性前列腺增生组织（22例），采用免疫组织化学SP法染色，检测BSP的表达情况。采用电化学发光法检测前列腺癌患者术前血清总前列腺特异性抗原（tPSA ）的含量。结果大多数前列腺癌高表达BSP，阳性率为76.47%（52／68），高于前列腺增生组织的13.64%（3／22）（χ2＝27.614，P＜0.001），周边正常腺组织无或低表达BSP。68例前列腺癌组织按Gleason分级系统进行病理分级，分化良好、分化中等和分化不良癌组织中BSP的表达率分别为75.0%（12／16）、77.5%（31／40）和75.0%（9／12），差异无统计学意义（χ2＝0.057，P＝0.972）。病理分期pT2、pT3、pT4癌组织中BSP的表达率分别为62.2%（23／37）、95.2%（20／21）和90.0%（9／10），差异有统计学意义（χ2＝9.338，P＝0.009）。BSP阳性前列腺癌患者的血清tPSA含量[（69.06±25.52）μg／L]高于BSP阴性患者[（38.00±21.64）μg／L]（F＝19.355，P＜0.001）。结论 BSP在前列腺癌中高表达，与病理分期、血清tPSA含量相关，可能与前列腺癌的生物学行为有关。

Objective To investigate the expression of bone sialoprotein （BSP） in prostate cancer and its clinical significance. Methods Prostate cancer tissues of different pathological grades （68 cases） and benign prostatic hyperplasia tissues （22 cases） were selected. SP method was used to detect the expression of BSP. Serum total prostate-specific antigen （tPSA） levels of prostate cancer were detected by electrochemiluminescence immunoassay before the operation. Results Compared with no or low expression in the adjacent normal glandular tissues, the detectable levels of BSP were examined in most of the prostate cancer tissues. The expression rate of BSP in prostate cancer tissues was higher than that in benign prostatic hyperplasia tissues [76.47%（52/68） vs 13.64%（3/22）,χ2=27.614, P〈0.001]. The expression rates of BSP in well differentiated, moderately differentiated and poorly differentiated tissues according to cell differentiating degree （Gleason system） were 75.0 % （12/16）, 77.5 % （31/40） and 75.0 % （9/12） respectively. There was no significant difference in various pathological grading （χ2=0.057, P=0.972）. The expression rates of BSP in pathological stage pT2, pT3 and pT4 tissues were 62.16%（23/37）, 95.24%（20/21） and 90.0%（9/10） respectively. A statistically significant association was found between BSP expression and pathological stage （χ2=9.338, P=0.009）. Serum tPSA level of prostate cancer group with BSP expression was higher than that with no BSP expression [（69.06±25.52）μg/L vs （38.00±21.64）μg/L, F=19.355, P〈0.001]. Conclusion The high expression of BSP in prostate cancer has a relationship with pathological stage and serum tPSA level, it may play an important role in the biological behaviour of prostate cancer.