肝胆外科患者感染与缝隙连接蛋白40及基质金属蛋白酶介导作用研究

Analysis of infections in department of hepatobiliary surgery and mediation of CONNEXIN40 and MMPs

目的分析肝胆外科患者术后感染的病原菌分布及耐药性,并探讨缝隙连接蛋白40(CONNEXIN40)及基质金属蛋白酶(MMPs)的参与作用,为临床治疗提供参考。方法选择医院2010年2月-2014年2月诊治的1 654例行肝胆外科手术患者临床资料,并对肝胆外科术后感染的80例患者进行研究,分析送检样本检出的病原菌种类、耐药性,检测感染与未感染患者血清中CONNEXIN40及基质金属蛋白酶2(MMP2)和基质金属蛋白酶9(MMP9)的水平变化。结果 80例感染患者共分离出病原菌95株,其中革兰阳性菌38株占40.0%,革兰阴性菌57株占60.0%;革兰阴性菌对庆大霉素耐药率>50.0%,革兰阳性菌对青霉素G耐药率较高为63.2%,感染患者血清中MMP2、MMP9及CONNEXIN40水平增高。结论革兰阴性菌是肝胆外科患者术后感染的主要病原菌,且肝胆外科术后感染可能与患者CONNEXIN40及MMPs水平的异常相关。

OBJECTIVE To analyze the pathogen distribution and drug resistance of postoperative infections in the department of hepatobiliary surgery and the mediation of CONNEXIN40 and MMPs so as to provide reference for clinical treatment .METHODS Totally 80 cases of infected patients in our hospital were enrolled in the present stud‐y .The pathogenic bacterial types and drug resistance as well as the expression of CONNEXIN40 combined MMP2/9 were assayed .RESULTS Totally 95 strains of bacteria were isolated from the patients ,including 38 gram‐positive strains （40 .0% ） and 57 gram‐negative strains （60 .0% ） .Gram‐negative strains had a resistance of〉 50 .0% to gentamicin and gram‐positive strains had a resistance up to 63 .2% to penicillin .The serum MMP2 , MMP9 and CONNEXIN40 were increased in infected patients .CONCLUSION Gram‐negative bacteria are the main type resulting infections in patients and this process has a close relationship with abnormal level of serum CON‐NEXIN40 and MMPs concentration .