树脂柱层析法分离纯化籽瓜中L-瓜氨酸

Isolation and purification of L-citrulline in seeding watermelon by resin column chromatography

目的：研究适合分离纯化籽瓜L-瓜氨酸的树脂柱层析方法。方法：选择001×7、D001和D061三种阳离子交换树脂进行L-瓜氨酸静态吸附解析和动态平衡实验，优化分离提纯籽瓜L-瓜氨酸工艺。通过考察温度、pH、浓度、吸附穿透曲线及洗脱液浓度、流速因素，进一步研究001x7树脂层析柱分离纯化籽瓜L-瓜氨酸的条件。测定三种不同极性大孔吸附树脂和活性炭对籽瓜提取液的脱色率和吸附率，优化脱色工艺。结果：分离提纯籽瓜L-瓜氨酸工艺用001×7型阳离子交换树脂较适合，最佳工艺条件：上柱温度为40—50℃，上柱液的pH为3，洗脱液选择0．5mol／L的氨水，洗脱流速为O．5mL／min，洗脱液的用量为6倍树脂体积。脱色工艺使用HZ-801大孔吸附树脂较合适，脱色精制后可得到纯度为96．6％的L-瓜氨酸产品。结论：001×7型阳离子交换树脂层析柱和HZ-801大孔吸附树脂相结合，可以有效分离纯化籽瓜中的L-瓜氨酸，对充分利用籽瓜资源和改变我国L-瓜氨酸成本较高现状具有十分重要的意义。

Objective：Isolation and purification of L-citrulline in seeding watermelon by resin column chromatography were studied. Method=The behavior of static adsorption,desorption and dynamic balance experiments of three kinds of cation exchange resin column was compared. The 001x7,D001 and D061 resins were employed to separate and purify the L-citrulline extracts from seeding watermelon. By investigating the temperature,pH,concentration,adsorption breakthrough curve,concentration of eluent and flow rate,further study on 001x7 column chromatography separation and purification of L-citrulline condition was discussed. By determination decolorization rate and adsorption rate of three different polar macroporous adsorption resins and activated carbon,the decolorization technology of extracts from seed watermelon was optimized. Results= It＇s the best to use 001 x7 resin to adsorb and purify L-citrulline from seeding watermelon. The optimal purification conditions were as follows.temperature of 40-50℃,pH 3,velocity of 0.5 mol/L ammonia taken as eluent 0.5 mL/min,and volume of ammonia water 6 BV （bed volume）. Decolorization technology using HZ-801 macroporous adsorption resin was better and the purity of L-citrulline could reach up to 96.6% after further recrystallization. Conclusion：The combination of 001 x7 resin column chromatography and HZ-801 macroporous resin for separation and purification of seed watermelon L-citrulline was an effective method. The research had the significance meaning for fully using of seed watermelon resources and reducing high cost of L-citrulline in our country current situation.