水溶性红曲色素液态发酵培养基成分优化

Optimization of liquid-state fermentation culture medium for watersoluble monascus pigment

对ACCC 30352(GIM 3.439)紫红曲霉液态发酵培养基成分进行筛选,发现葡萄糖、蔗糖、麦芽糖、可溶性淀粉、有机氮源、Mg2+、Zn2+、Mn2+、Fe2+、甘氨酸有利于色素合成;最适碳源含量为5%;最适碳氮比(质量比)为1:1。在此基础上,通过正交试验对碳源、氮源和碳氮比(质量比)作进一步分析,确定其液态发酵代谢水溶性色素的最佳培养基成分为:蔗糖5%、酵母膏1.07%、Na NO3 0.1%、甘氨酸0.5%、Mg SO4 0.05%、Zn SO4 0.001%、Mn SO4 0.003%、Fe SO40.001%。

The liquid-state fermentation culture media for water-soluble monascus pigment by Monascus purpureus ACCC 30352（GIM 3.439） were optimized, and the resulted showed that glucose, sucrose, maltose, soluble starch, organic nitrogen source, Mg2＋, Zn2＋, Mn2＋, Fe2＋, and glycine were advantageous to the biosynthesis of Monascus pigment; and the optimum carbon content and C：N（mass ratio） was, respectively, 5% and 1：1. Based on this fact, the effect of carbon, nitrogen and C：N（mass ratio） were further researched with orthogonal array design. As a result, the perferable fermentation culture medium was as follows： sucrose 5%, yeast extract 1.07%, Na NO3 0.1%, glycine 0.5%, Mg SO4 0.05%, Zn SO4 0.001%, Mn SO40.003%, Fe SO4 0.001%.