摘要
对ACCC 30352(GIM 3.439)紫红曲霉液态发酵培养基成分进行筛选,发现葡萄糖、蔗糖、麦芽糖、可溶性淀粉、有机氮源、Mg2+、Zn2+、Mn2+、Fe2+、甘氨酸有利于色素合成;最适碳源含量为5%;最适碳氮比(质量比)为1:1。在此基础上,通过正交试验对碳源、氮源和碳氮比(质量比)作进一步分析,确定其液态发酵代谢水溶性色素的最佳培养基成分为:蔗糖5%、酵母膏1.07%、Na NO3 0.1%、甘氨酸0.5%、Mg SO4 0.05%、Zn SO4 0.001%、Mn SO4 0.003%、Fe SO40.001%。
The liquid-state fermentation culture media for water-soluble monascus pigment by Monascus purpureus ACCC 30352(GIM 3.439) were optimized, and the resulted showed that glucose, sucrose, maltose, soluble starch, organic nitrogen source, Mg2+, Zn2+, Mn2+, Fe2+, and glycine were advantageous to the biosynthesis of Monascus pigment; and the optimum carbon content and C:N(mass ratio) was, respectively, 5% and 1:1. Based on this fact, the effect of carbon, nitrogen and C:N(mass ratio) were further researched with orthogonal array design. As a result, the perferable fermentation culture medium was as follows: sucrose 5%, yeast extract 1.07%, Na NO3 0.1%, glycine 0.5%, Mg SO4 0.05%, Zn SO4 0.001%, Mn SO40.003%, Fe SO4 0.001%.
出处
《食品科技》
CAS
北大核心
2015年第9期252-257,共6页
Food Science and Technology
基金
四川省教育厅基础研究项目(14ZB0374)
四川省中医药管理局项目(2014G001)
关键词
红曲霉
液态发酵
色素
水溶性红曲色素
成分优化
monascus
liquid-state fermentation
pigment
water-soluble monascus pigment
composition optimization