以BMSCs为基础构建组织工程骨修复种植区骨量不足的实验研究

BMSCs-based tissue-engineering bone repairing bone defect in dental implant region: an experimental study

目的评价以骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)为基础构建组织工程骨修复种植体周围骨缺损的成骨效果。方法在3只犬双侧下颌前磨牙区各植入2颗种植体,分为对照组和实验组,每组6颗种值体;在种植体远中制备骨缺损,对照组每侧骨缺损区随机植入富血小板血浆(platelet-rich plasma,PRP)和实验组成骨诱导培养BMSCs复合PRP。分别于移植后1、3个月取材,行大体、影像学及组织学观察。结果 1个月后,实验组可见较多的骨基质,较少的胶原纤维。实验组骨密度值大于对照组(101±8.99)vs(93±9.81),差异有统计学意义(P<0.05)。3个月后,实验组可见较多的哈弗系统,实验组与对照组骨密度值变化不大(100.75±12.05)vs(105±11.58),差异无统计学意义。结论以BMSCs为基础的组织工程骨能在种植体周围较好地成骨。

Objective To repair bone defect in implant region using bone marrow mesenchymal stem cells-based tissue-engineering bone and investigate its osteogenesis potential. Methods Bilateral implantaion,two implants every side,was performed in the mandible premolar region of 3 Beagles and bone defects were created in the distal of every implant. Two bone defects in one side of mandible premolar region were randomly repaired with two types of grafts： PRP（ n = 6,the control group）; and compound osteogenicinduced BMSCs with PRP（ n = 6,the experimental group）. 1 and 3 months after transplation,gross investigation,X-ray examination and histological observation were conducted to examine bone formation. Results One month after implantation,the osteogenesis in experimental group was better than in the control group. We detected more bone matrix and less collagenous fibers in the experimental group. The bone density in the experimental group was higher than in the control group and the difference was significantly different[（ 101 ± 8. 99） vs（ 93 ± 9. 81）,P≤0. 05]. Three months after implantation,the bone density in the experimental group tended to be higher than in the control group; however,the difference was not statistically significant[（ 100. 75 ± 12. 05） vs（ 105 ± 11. 58） ]. But we detected more Haversian system in the experimental group. Conclusion Bone marrow stem cell-based tissue-engineered bone has high osteogenic potential around implants.