一测多评法同时测定舒血宁注射液中6种黄酮类成分

Simultaneous Determination of Six Flavonoid Ingredients in Shuxuening Injection by Quantitative Analysis of Multi-components by Single-marker

目的:采用一测多评(quantitative analysis of multi-components by single marker,QAMS)法,同时测定舒血宁注射液中6种黄酮类成分,并考察其方法的耐用性与系统适应性。方法:以舒血宁注射液为研究对象,以芦丁为内参物,建立芦丁与水仙苷、槲皮素-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、山奈酚-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、槲皮素-3-O-[2-O-(1-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷、山奈酚-3-O-[2-O-(6-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷间的相对校正因子(f),比较该方法与外标法测定结果的差异,并对该方法进行再验证。结果:利用相对校正因子计算舒血宁注射液中这6种黄酮类成分的含量,与外标法比较,差异较小(RSD<5%)。结论:QAMS可以作为一种简便准确的质量评价模式用于舒血宁注射液多种成分的定量测定,为后续质量标准评价研究提供了参考依据。

Objective：To simultaneous determine six flavonoids in Shuxuening injection by quantitative analysis of multi-compo-nents by single marker （ QAMS） and examine the durability and system suitability of the analysis. Methods：Shuxuening injection was the study object and rutin was used as the internal material, the relative correction factor （ f） between rutin and narcissus glycosides, quercetin-3-O-β-D-glucosyl （1-2）-α-L- rhamnoside, kaempferol-3-O-β-D-glucose group （1-2）-α-L-rhamnopyranoside, quercetin-3-O-[2-O-（1-O）-p-coumaric acid-β-D-glucose-yl]-α-L-rhamnoside, kaempferol-3-O-[2-O-（6-O）-on coumaroyl-β-D-glucose-based]-α-L-rhamnosides was respectively established. The result differences between the internal standard method and external standard method were compared, and the internal standard method was re-validated. Results： The contents of the six flavonoids in Shuxuening injection were calculated by the correction factors. Compared with those of the external standard method, the differences in the contents of the method were small（ RSD〈5%） . Conclusion：QAMS can be used as a simple and accurate quantitative quality evaluation meth-od for the determination of a variety of ingredients in Shuxuening injection, which provides reference for the subsequent study on quality standard.