摘要
目的:建立胃灵颗粒的质量标准。方法:采用TLC法对胃灵颗粒中的主要药味白芍、甘草、延胡索及山楂进行定性鉴别;采用HPLC法测定白芍中芍药苷的含量,色谱柱:Agilent XDB-C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸溶液(10∶90),检测波长:230 nm,流速:1.0 ml·min-1。结果:薄层色谱斑点清晰,在与对照药材或对照品相应的位置上显相同颜色的斑点,阴性对照无干扰;白芍中芍药苷的线性范围0.151-1.212μg,r=0.999 9,平均回收率为99.63%,RSD为2.01%(n=9)。结论:本方法操作简单,结果准确可靠,重复性好,可作为胃灵颗粒的质量控制方法。
Objective:To re-establish the quality control standard for Weiling granules. Methods:The 4 chief herbs in the prepa-ration, radices paeoniae alba, licorice, rhizoma corydalis and hawthorn were identified by TLC qualitatively. The content of paeoniflor-in in radices paeoniae alba was determined by HPLC. The separation was performed on an Agilent XDB-C18 (250 mm × 4. 6 mm, 5μm)coulme with mobile phase consisting of acetonitrile-0. 1% phosphoric acid solution (10:90). The detection wavelength was 230 nm and the flow rate was 1. 0 ml·min-1 . Results:The spots in TLC were clear without any interference. The linear range for paeoni-florin was 0. 151-1. 212 μg(r=0. 999 9,n=5). The average recovery was 99. 63% and RSD was 2. 01%(n=9). Conclusion:The method is simple and accurate with high reproducibility, which can be used for the quality control of Weiling granules.
出处
《中国药师》
CAS
2015年第10期1720-1723,共4页
China Pharmacist
基金
军队医疗机构制剂标准提高科研专项课题(编号:13ZJZ03)