三七活血片治疗急性软组织损伤的机制探讨

Mechanism Exploration of Sanqi Huoxue Pills in the Treatment of Acute Soft Tissue Injury

目的:研究三七活血片对急性软组织损伤的治疗作用及其机制。方法:140只大鼠分为空白组,模型组,阳性药对照组,三七活血片低、中、高剂量组(0.5,1.0,2.0 g·kg-1),建立大鼠急性软组织损伤模型,于给药d3和d5,取大鼠损伤组织测定前列腺素(PGE2)、白细胞介素-6(IL-6)、一氧化氮(NO)及超氧化物歧化酶(SOD)含量;给药d5,观察三七活血片对大鼠全血黏度、血浆黏度的影响。结果:与模型组比较,三七活血片可明显降低大鼠受伤组织中NO、PGE2与IL-6水平;升高组织中SOD水平;可明显降低血液黏度和血浆黏度(P<0.01或P<0.05)。结论:三七活血片对大鼠急性软组织损伤有较好的治疗和修复作用,其治疗机制可能与抑制受伤局部组织细胞释放的自由基、PGE2、IL-6的含量,提高局部血液流变有关。

Objective： To explore the effect and mechanism of SanQi HuoXue pills in the treatment of acute soft tissue injury. Methods： Totally 140 wistar rats were randomly divided into the control group, model group, posistive drug group, and Sanqi Huoxue pills group respectively at the dose of 0.5,1.0,2.0 g · kg-1. The rat model of acute soft tissue injury was used to observe the influence of Sanqi Huoxue pills on the content of PGE2, IL-6, NO and SOD on the 3rd and 5th day after the administration, and the effects of the pills on the whole blood viscosity and plasma viscosity were studied as well on the 5th day. Results： After the administration of the pills, the levels of PGE2, IL-6 and NO, the blood viscosity and plasma viscosity were decreased significantly, and the level of SOD was increased significantly（ P 〈 0.01 or P 〈 0.05 ） when compared to model group. Conclusion： Sanqi Huoxue pills have therapeutic effect on acute soft tissue injury in rats. Free radical, PGE2, IL-6 and blood hemorheology play important roles in the formation of acute soft tissue injury.