HPLC法同时测定祛喘胶囊中木兰脂素、欧前胡素、异欧前胡素的含量

Simultaneous Determination of Imperatorin,Isoimperatorin and Magnolin in Quchuan Capsules by HPLC

目的：建立祛喘胶囊中对木兰脂素、欧前胡素和异欧前胡素的HPLC测定方法。方法：采用日本资生堂CAPCELL PAK C18色谱柱（150 mm×4.6 mm,5μm）,以乙腈-水为流动相进行梯度洗脱,柱温为30℃,流速为1.0 ml·min-1,检测波长在0-25 min为278 nm,25-70 min为248 nm,进样量为10μl。结果：木兰脂素在6.126-55.134μg·ml-1范围内线性关系良好（r=0.999 7）,平均回收率为101.5%,RSD为1.4%;欧前胡素在9.862-88.758μg·ml-1范围内线性关系良好（r=0.999 8）,平均回收率为99.6%,RSD为1.2%;异欧前胡素在4.830-43.470μg·ml-1范围内线性关系良好（r=0.999 8）,平均回收率为100.2%,RSD为1.7%。结论：本法操作简便、结果准确,可用于祛喘胶囊的质量控制。

Objective. To develop a method to simultaneously determine the content of imperatorin, isoimperatorin and magnolio in Quchuan capsules by HPLC. Methods： A Shiseido CAPCELL PAK C18 column（ 150 mm × 4.6 mm,5 μm） was used. The mobile phase consisted of acetonitrile（A） - water（B） with gradient elution at a flow rate of 1.0 ml · min -1 and the column temperature was at 30℃. The detection wavelength was set at 278 nm from 0 to 25 min and 248 nm from 25 to 70 min. The injection volume was 10 μl. Results： The linear range of imperatorin,isoimperatorin and magnolin was 6. 126-55. 134 μg · ml-1 （ r =0. 999 7 ）, 9. 862-88. 758 μg · ml-1 （r =0.999 8） and 4.830-43.470 μg · ml-1 （r =0.999 8）, respectively. The average recovery was 101.5% （RSD = 1.4% ）, 99.6% （RSD = 1.2% ） and 100.2% （RSD = 1.7% ）, respectively. Conclusion ： The method is convenient, sensitive and accurate, which can be used for the quality control of Quchuan capsules.