豚鼠主动全身过敏试验两种阳性物质选择的探讨

Exploration on the selection of positive materials in active systemic anaphylaxis in guinea pigs

目的比较两种阳性物质人血白蛋白、卵白蛋白对豚鼠主动全身过敏作用,为过敏性试验提供较好的阳性对照。方法将豚鼠随机分为14组,以人血白蛋白、卵白蛋白（2、10、100 mg/只）、0.9%氯化钠注射液等受试物为对照,研究不同致敏剂量、激发剂量、激发时间等条件下,豚鼠全身主动过敏的反应情况。结果在2-100mg/只剂量范围内,人血白蛋白、卵白蛋白豚鼠主动全身过敏反应的发生率为100%。在2-10 mg/只剂量范围内,过敏症状发生程度随致敏剂量、激发剂量的增加而增加,相同剂量的卵白蛋白较人血白蛋白反应程度更强。结论豚鼠主动全身过敏试验,阳性对照推荐使用卵白蛋白,剂量为2 mg/只。

Objective The aim of this study was to provide a better positive control for allergic test by comparing the allergic effect of two kinds of positive materials,human albumin and ovalbumin,on active systemic anaphylaxis in guinea pig. Methods Guinea pigs were randomly divided into 14 groups,and were given human albumin,ovalbumin（ 2,10,100 mg/animal）,or 0. 9% sodium chloride injection as test substances,to assess the symptoms and incidence of systemic allergic responses induced by different sensitizing substances in different challenge doses and different challenge intervals. Results In the range of 2 to 100 mg / animal,the guinea pigs showed a 100% incidence rate of positive allergic reaction to human albumin and ovalbumin,the severity of anaphylactic symptoms was increasing along with the increase of sensitizing doses and challenge doses,and the allergic reaction was more strong induced by the same dose of ovalbumin than human albumin. Conclusions Our findings indicate that in the active systemic anaphylaxis test in guinea pigs,we recommend ovalbumin as the positive control in a dose of 2 mg / animal.