层粘连蛋白在良恶性甲状腺肿瘤组织中的表达及意义

Expression of Laminin in Thyroid Adenoma and Thyroid Carcinoma

目的 :探讨层粘连蛋白 (LN)在良恶性甲状腺肿瘤的表达及其意义。方法 :采用免疫组化方法检测 10例甲状腺正常组织和 4 6例甲状腺腺瘤、19例甲状腺癌组织中LN的表达 ,对LN表达率两两比较。结果 :LN在甲状腺瘤与甲状腺癌组织中阳性表达率差异有显著性意义 (P <0 .0 5 ) ;在甲状腺癌组织中与甲状腺正常组织中的阳性表达率差异有高度显著性 (P <0 .0 1)。LN在甲状腺癌细胞及出现于甲状腺滤泡性腺瘤组织中的乳头状腺瘤细胞胞浆中有表达。结论 :LN参与了肿瘤的发生发展与癌变 ,可作为甲状腺良恶性肿瘤的鉴别诊断指标之一。

Objective: To investigate the role of laminin (LN) in the turnover of thyroid from adenoma to carcinoma,and detect the expression level of LN in the normal, adenoma and carcinoma tissue of thyroid. Methods: Expression of LN in 10 cases normal thyroid and 46 cases thyroid adenomas, 19 cases thyroid carcinomas were investigated by s p method immunohistochemistry. Results: In the tissue of carcinoma of thyroid, the positive rate of LN protein expression was much higher than that in the thyroid adenoma (P<0.05), and than that in the normal tissue (P<0.01). Positive immunostaining of LN also was found in the cytoplasm of tumor cells of carcinoma and papillary hyperplasia appeared in the follicular adenoma. Conclusion: LN exists in occurrence and development of thyroid tumors, and can be taken as a marker of malignant thyroid neoplasm. LN expression in the cytoplasm of tumor cells of carcinoma and papillary hyperplasia in the follicular adenoma may indicate the malignant transformation.