乳腺癌患者血浆中miR-127-3p表达的临床价值

The clinical value of miR-127-3p expression in plasma of patients with breast cancer

目的探讨血浆miR-127-3p在乳腺癌患者中的表达及其临床意义。方法收集南通大学附属医院2013年10月至2014年1月收治的80例乳腺癌患者、70名良性乳腺肿瘤患者及70名健康体检者血浆标本，采用SYBRGreen实时荧光定量逆转录聚合酶链反应（RT．PCR）检测血浆中miR-127-3p含量，评价检测方法的线性、重复性及特异性，并对乳腺癌患者血浆miR-127-3p含量与癌胚抗原（CEA）、CAl53浓度进行相关性分析。Mann—Whitney检验分析血浆miR-127-3p的表达与乳腺癌患者临床病理特征的关系。结果成功建立血浆miR-127-3p的检测方法，乳腺癌患者血浆中miR-127-3p的相对表达量（RQ）14．158（5．424～16．465）显著高于乳腺良性肿瘤患者3．015（1．987～5．035）（P=0．0006）和健康对照者2．375（1．173～4．370）（P=0．0002）。而乳腺良性肿瘤患者与健康对照者之间，差异无统计学意义（P=0．143）。miR-127-3p相对表达量与CA153浓度正相关性（R^2=0．457，P=0．003）。miR-127-3pROC曲线下面积（AUC）为0．763，高于传统乳腺肿瘤标志物CEA和CA153。乳腺癌患者血浆miR-127-3p的表达水平在不同肿瘤直径、分化程度和TNM（tumornodemetastasis）分期间差异无统计学意义（P〉0．05）。结论miR-127-3p在乳腺癌患者中高表达，可能是诊断乳腺癌患者的一个重要参考指标。

Objective To investigate the expression and clinical value of miR-127-3p in plasma of patients with breast cancer. Methods 80 cases of breast patients, 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited. A real-time fluorescent quantitative reverse transcription polymerase chain reaction （RT-PCR） method for detecting miR-127-3p was established; Liner, reproducibility, and specificity were evaluated. In addition, correlations between the relative expression of plasma miR-127-3p and the concentrations of CEA and CA153 were assessed, the relationship of miR-127- 3p expression and clinicopathological features was further determined by Mann-Whitney test. Results The method for detection of plasma miR-127-3p was established. The relative plasma expression of miR-127-3p in breast patients [ 10. 561 （ 5.424 - 16. 465 ） ] was significantly higher than that in benign breast tumor patients [3. 015 （ 1. 987 -5. 035） ] （P =0. 000 6） and healthy controls [2. 375 （ 1. 173 -4. 370） ] （P = 0. 000 2）. However, there was no significant difference between benign tumor patients and healthy control group （P = 0. 143 ）. Positive relationship was found between the relative expression of miR-127-3p and the concentration of CA153 （ R^2 = 0. 457, P = 0. 003 ）. The area under the ROC curve （AUC） of miR-127-3pwas 0. 763, which was higher than that of CEA and CA153. No significant difference was found between plasma miR-127-3p expression and clinicalpathological features including tumor size, differentiation and tumor node metastasis stage （P 〉 0.05 ）. Conclusions miR-127-3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer.