重组合异体冻干骨修复兔桡骨节段性骨缺损

Bone Repaired by Reconstituted Freeze-Dried Bone Allograft in Segmental Defects of Radius in Rabbits

目的 :研究碱性成纤维细胞生长因子 (bFGF)和透明质酸凝胶 (HAG)复合异体冷冻干燥骨修复兔桡骨缺损的能力 ,并探讨它们的作用机制。方法 :于 36只新西兰大白兔两侧桡骨干处造成 15mm缺损 ,采用 3种不同的治疗方法 :A组 ,植入bFGF ,HAG与冻干骨的复合物 ;B组 ,植入吸附bFGF的冻干骨 ;C组 ,单纯植入冻干骨作为对照。在术后不同时间进行放射学、组织学和核素扫描检查 ,并测定钙含量。结果 :A组在术后不同时间的骨代谢活性 ,新骨生成量和钙含量均高于B组 ,B组高于C组 (P <0 .0 5或P <0 .0 1)。A组和B组缺损分别于术后 8周和 10周完全愈合。结论 :bFGF作为一种骨生长因子 ,促进新骨生成 ;HAG作为缓释载体 ,提高bFGF的效能 。

Objective: To study the effect of basic fibroblast growth factor(bFGF) and hyaluronic acid gel (HAG) combined with freeze dried bone allograft in repairing radius defects and explore their mechanism. Methods: 15 mm segmental bone/periosteum defects were created in 36 New Zealand rabbits on bilateral radius and were treated with three different kinds of implants: A,bFGF and HAG combined with freeze dried bone were implanted; B,bFGF combined with freeze dried bone; C, a single freeze dried bone as control. The repairs of defects were observed radiologically, histologically and were analyzed by radionuclide bone imaging and measurement of calcium contents at different periods. Results: The new bone formation, bone metabolic activity and calcium contents of defects in Group A were higher than that in Group B, and the data of Group B were higher than that in Group C(P<0.05 or P<0.01). The defects of Group A were healed at the 8th week, and those of Group B were healed at the 10th week. Conclusion: As a osteogenetic factor, bFGF promotes the new bone formation. As a slow release carrier, HAG enhances the effectiveness of bFGF. The combination of bFGF, HAG and freeze dried bone allograft can repair the defects more effectively.