高效液相色谱法测定脑脉康颗粒中黄芪甲苷含量

Determination of Astragaloside Ⅳ in Naomaikang Granules by HPLC

目的建立测定脑脉康颗粒君药黄芪中黄芪甲苷含量的高效液相色谱（HPLC）法。方法色谱柱为AgilentC18柱（250mm×4．6mm，5μm），柱温为30℃，流动相为乙腈-水（36：64），流速为1mL／min。结果黄芪甲苷进样质量浓度线性范围是0．0354～0．3542g／L，线性回归方程为logA=1．6020logCi＋3．0221，r2=0．9999（n=8）；平均回收率为102．60％，RSD：2．96％（n=6）。结论该方法简便、准确、专属性强，可用于测定脑脉康颗粒君药黄芪中黄芪甲苷的含量。

Objective To establish a method for quality control of Naomaikang Granules. Methods The chromatography column was Agi- lent C18（250 mm×4. 6 mm,5 μm）.The mobile phase consisted of Acetonitrile - water（36：6d）at a flow rate of 1 mL/min,at 30 ℃. Results Astragaloside Ⅳ showed a good linear relationship within the range of 0. 0354mg/mE-0. 354 2 mg/mL,equation of linear re- gression was logA = 1. 602 0 logCi ＋3. 022 1, R2 =0. 999 9（n =8）, the average recovery was 102.60% , RSD was 2.96% （n =6）. Conclusion The methods are simple ,exact and reproducible, and can be used for the qualitative and quantitative control of Naomaikang Granules.