PPARα、γ配体对单核细胞来源的巨噬细胞的作用观察

Effects of PPARα and γ ligands on monocyte-derived macrophages

目的 探讨PPARα和γ配体在动脉粥样硬化病变局部的作用。方法 观察PPARα和γ配体对氧化低密度脂蛋白 (ox LDL)介导的巨噬细胞清道夫受体A(SRA)基因表达、肿瘤坏死因子 (TNF α)和基质金属蛋白酶 9(MMP 9)释放、细胞增殖与凋亡的影响。结果 中低浓度的PPARα和γ配体不但增强ox LDL介导的巨噬细胞TNF α、MMP 9表达 ,而且有促细胞凋亡和抗细胞增殖的作用 ;PPARγ配体不但能显著增加巨噬细胞PPARγmRNA表达 ,而且还能抑制ox LDL介导的巨噬细胞SRA。结论 PPARα和γ基因激活可能有抗炎和抗细胞增生的作用 ,后者可能还有助于减少粥样斑块脂质内容。

Objective To probe the roles of the ligands of peroxisome proliferator activated receptor α (PPARα) and PPARγ in the pathogenesis of atheroma lesion. Methods The effects of PPARα and γ ligands on expressions of scavenger receptor A (SRA) gene, release of cytokines (TNF α and MMP 9) and the cell growth and apoptosis induced by ox LDL were analyzed. Results (1)Both PPARα and γ ligands not only promoted macrophage cells apoptosis and inhibited the cells growth, but also decreased the expression of TNF α and MMP 9 protein induced by ox LDL; (2)PPARγligand not only significantly lowered macrophages SRA gene expression induced by ox LDL, but also increased macrophages PPARγ mRNA expression , while PPARαgene expression had no effects of SRA gene expression induced by ox LDL. Conclusion PPARαand PPARγ gene translation may suppress cell growth and inflammatory reaction, and the latter may be helpful for lowering lipid contents of atheromatous plaque.