川芎硫磺熏蒸前后的傅里叶变换红外光谱鉴别

Identification of Sun-dried and Sulfur-fumigated Chuanxiong Rhizoma by FTIR

目的：评价硫磺熏蒸对川芎化学成分的影响。方法：运用傅里叶变换红外光谱法对川芎硫磺熏蒸前后的醇提物及水提物进行分析。红外光谱图采用Omnic 8.0软件采集;二阶导数图谱采用OPUS 6.5软件对红外光谱图进行平滑及求导处理;吸收波数采用SPSS软件进行主成分分析。结果：川芎经过硫磺熏蒸之后,在波数2 000-750 cm-1区间内变化明显,某些吸收峰削弱或消失,同时也有一些新的吸收峰出现。结论：硫磺熏蒸对川芎的化学成分有一定的影响,应用傅里叶变换红外光谱法并结合二阶导数和主成分分析可有效、直观、快速地鉴别硫熏和非硫熏川芎。

Objective： To evaluate the sulfur-fumigation effects on the chemical constituents of Chuanxiong Rhizoma. Methods： Alcoholic and aqueous extracts of sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples were analyzed by FTIR. FTIR spectra were acquired and disposed by software Omnic 8. 0,second derivative IR spectra were analyzed by software OPUS 6. 5 through smoothing and differentiation treatment to FTIR spectra,and the absorption frequencies of alcoholic and aqueous extracts of sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples were assessed by using principal component analysis with software SPSS. Results： There were significant differences between sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples in the region ranging from 2 000 cm- 1to 750cm- 1. Some absorption peaks weakened or disappeared,and some newly emerged after the processing of sulfur-fumigation. Conclusion：The processing of sulfur-fumigation can influence the effective constituents of Chuanxiong Rhizoma. The established method of FTIR combined with second derivative and principal component analysis has been proved to be an effective,intuitional and rapid approach to distinguish sun-dried and sulfur-fumigated Chuanxiong Rhizoma.